PDE4D Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PDE4D Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

22

Rezensionen (1)

Kat-Nr. : 12918-1-AP

Synonyme

PDE43, EC:3.1.4.53, DPDE3, cAMP-specific phosphodiesterase 4D, 3',5'-cyclic-AMP phosphodiesterase 4D

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Galerie der Validierungsdaten

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  WB analysis using 12918-1-AP

  K562 cell lysate were subjected to SDS PAGE followed by western blot with 12918-1-AP (PDE4D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12918-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12918-1-AP (PDE4D antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 12918-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12918-1-AP (PDE4D antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse colon using 12918-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12918-1-AP (PDE4D Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse colon using 12918-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12918-1-AP (PDE4D Antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 12918-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PDE4D antibody (12918-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Maushirngewebe, Maus-Pankreasgewebe
• Erfolgreiche Detektion in IHC: Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 19 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Produktinformation

12918-1-AP bindet in WB, IHC, IF/ICC, ELISA PDE4D und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDE4D fusion protein Ag3588
• Vollständiger Name: phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila)
• Berechnetes Molekulargewicht: 809 aa, 91 kDa
• Beobachtetes Molekulargewicht: 91 kDa
• GenBank-Zugangsnummer: BC036319
• Gene symbol: PDE4D
• Gene ID (NCBI): 5144
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDE4D(cAMP-specific 3',5'-cyclic phosphodiesterase 4D) is also named as DPDE3, PDE43 and belongs to the cyclic nucleotide phosphodiesterase family and PDE4 subfamily. PDE4D subtype may be of particular importance as an antidepressant target in that it is regulated by repeated treatment with both norepinephrine and serotonin reuptake inhibitors as well as by the PDE4 inhibitor rolipram(PMID:16730340). It has some isoforms and all the PDE4D isoforms are expressed in healthy adult human hippocampus and in hippocampus from a patient with advanced Alzheimer disease(PMID:17353396).

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; FGF1 and insulin control lipolysis by convergent pathways.; Authors - Gencer Sancar
human	WB	Acta Pharm Sin B; Targeting PDE4 as a promising therapeutic strategy in chronic ulcerative colitis through modulating mucosal homeostasis.; Authors - Heng Li
mouse	WB	Cell Rep; miR-199a Links MeCP2 with mTOR Signaling and Its Dysregulation Leads to Rett Syndrome Phenotypes.; Authors - Keita Tsujimura
human	WB	JCI Insight; Inhibition of phosphodiesterase 4D suppresses mTORC1 signaling and pancreatic cancer growth; Authors - Mi-Hyeon Jeong
	WB	Diabetes; Salt inducible kinase 1 terminates cAMP signaling by an evolutionarily conserved negative feedback loop in beta cells.; Authors - Min-Jung Kim
human	WB,IHC,IF	Cancer Lett; PDE4D binds and interacts with YAP to cooperatively promote HCC progression.; Authors - Huili Ren ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

RICHA (Verified Customer) (10-09-2019)

Great and specific

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Protein from fibroblasts

PDE4D Antibody Western Blot, validation (1:500 dilution) in Protein from fibroblasts (Cat no:12918-1-AP)