PDE1A Polyklonaler Antikörper

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• KD/KO Validated

PDE1A Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 12442-2-AP

Synonyme

61 kDa Cam PDE, Cam PDE 1A, hCam 1, HCAM1, HSPDE1A, PDE1A

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Galerie der Validierungsdaten

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  WB analysis using 12442-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12442-2-AP (PDE1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12442-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12442-2-AP (PDE1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12442-2-AP

  IP result of anti-PDE1A (IP:12442-2-AP, 4ug; Detection:12442-2-AP 1:1000) with mouse brain tissue lysate 2080 ug.

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  IHC staining of mouse brain using 12442-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12442-2-AP (PDE1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12442-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12442-2-AP (PDE1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 12442-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12442-2-AP (PDE1A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 12442-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12442-2-AP (PDE1A antibody) at dilution of 1:50 (under 25x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

12442-2-AP bindet in WB, IP, IHC, ELISA PDE1A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDE1A fusion protein Ag3157
• Vollständiger Name: phosphodiesterase 1A, calmodulin-dependent
• Berechnetes Molekulargewicht: 545 aa, 61 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: BC022480
• Gene symbol: PDE1A
• Gene ID (NCBI): 5136
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDE1A(Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A) is also named as Cam-PDE 1A, 61 kDa Cam-PDE, hCam-1 and belongs to the cyclic nucleotide phosphodiesterase (PDEs) family. The PDEs play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. This protein has 9 isoforms produced by alternative splicing with the molecular weight from 57 kDa to 63 kDa. There is a report showing the major 67-kD PDE1A isoform identified in human sperm associates tightly with calmodulin and is not activated by Ca(2+)/calmodulin(PMID:12135876).

Publikationen

Species	Application	Title
		Biol Reprod; Luteinizing Hormone Causes Phosphorylation and Activation of the cGMP Phosphodiesterase PDE5 in Rat Ovarian Follicles, Contributing, Together with PDE1 Activity, to the Resumption of Meiosis.; Authors - Jeremy R Egbert
mouse	WB	Biol Reprod; Follicle-stimulating hormone and luteinizing hormone increase Ca2+ in the granulosa cells of mouse ovarian follicles†.; Authors - Jeremy R Egbert
mouse	WB	PLoS One; Generation and phenotypic characterization of Pde1a mutant mice.; Authors - Xiaofang Wang ; KO Validated
		Cell Rep; Single-Cell Analysis of Foxp1-Driven Mechanisms Essential for Striatal Development.; Authors - Ashley G Anderson
human	IHC	Front Genet; Transcriptome profiling of intact bowel wall reveals that PDE1A and SEMA3D are possible markers with roles in enteric smooth muscle apoptosis, proliferative disorders, and dysautonomia in Crohn's disease; Authors - Yun Yang