PDCD4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PDCD4 Polyklonal Antikörper für WB, IP, IHC, ELISA, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, ELISA, FC (Intra)

Konjugation

Unkonjugiert

Publikationen

32

Kat-Nr. : 12587-1-AP

Synonyme

H731, Nuclear antigen H731 like, PDCD4, Protein 197/15a

---

Galerie der Validierungsdaten

• 

  WB analysis using 12587-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12587-1-AP (PDCD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK293 cells using 12587-1-AP

  WB result of PDCD4 antibody (12587-1-AP, 1:4000) with si-control and si-PDCD4 transfected HEK293 cells.

• 

  WB analysis of MCF-7 using 12587-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12587-1-AP (PDCD4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12587-1-AP

  IP result of anti-PDCD4 (IP:12587-1-AP, 4ug; Detection:12587-1-AP 1:3000) with HeLa cells lysate 4000ug.

• 

  IHC staining of human breast cancer using 12587-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12587-1-AP (PDCD4 antibody) at dilution of 1:50 (under 10x lens).

• 

  FC experiment of MCF-7 using 12587-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human PDCD4 (12587-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HEK-293-Zellen, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 28 publications below
• IHC: See 8 publications below
• IF: See 2 publications below

Produktinformation

12587-1-AP bindet in WB, IP, IF, IHC, ELISA, FC (Intra) PDCD4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDCD4 fusion protein Ag3288
• Vollständiger Name: programmed cell death 4 (neoplastic transformation inhibitor)
• Berechnetes Molekulargewicht: 469 aa, 52 kDa
• Beobachtetes Molekulargewicht: 54-64 kDa
• GenBank-Zugangsnummer: BC026104
• Gene symbol: PDCD4
• Gene ID (NCBI): 27250
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation, progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A. The calculated molecular wight of unmodified PDCD4 protein is 52 kDa, but the modified protein is about 54-64 kDa.

Publikationen

Species	Application	Title
human	WB,IHC	J Nanobiotechnology; Cold exposure protects against medial arterial calcification development via autophagy; Authors - Fu-Xing-Zi Li
human	WB	Oncogene; miR-21a in exosomes from Lewis lung carcinoma cells accelerates tumor growth through targeting PDCD4 to enhance expansion of myeloid-derived suppressor cells.; Authors - Xingju Zhang ; KD Validated
mouse	WB,IHC	Clin Transl Med; Phosphoproteome reveals molecular mechanisms of aberrant rhythm in neurotransmitter-mediated islet hormone secretion in diabetic mice.; Authors - Yunqiang He
rat	WB	Biomed Pharmacother; MiR-208a-3p aggravates autophagy through the PDCD4-ATG5 pathway in Ang II-induced H9c2 cardiomyoblasts.; Authors - Li Wang ; KD Validated
rat,mouse	WB,IHC,IF	Oxid Med Cell Longev; Protective Effects of PPARγ on Renal Ischemia-Reperfusion Injury by Regulating miR-21; Authors - Ruizhen Huang
		Cell Cycle; Resveratrol prevents rapamycin-induced upregulation of autophagy and selectively induces apoptosis in TSC2-deficient cells.; Authors - Anya Alayev