PD-ECGF Polyklonaler Antikörper

PD-ECGF Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF, FC, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 12383-1-AP

Synonyme

ECGF1, Gliostatin, hPD ECGF, MNGIE, PD ECGF, PDECGF, PD-ECGF, TdRPase, thymidine phosphorylase, TP, TYMP

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Galerie der Validierungsdaten

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  WB analysis using 12383-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12383-1-AP (PD-ECGF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 12383-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12383-1-AP (PD-ECGF antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12383-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12383-1-AP (PD-ECGF antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 12383-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12383-1-AP (PD-ECGF antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 12383-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12383-1-AP (PD-ECGF antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12383-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12383-1-AP (PD-ECGF antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Magenkrebsgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Produktinformation

12383-1-AP bindet in WB, IF, FC, IHC, ELISA PD-ECGF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PD-ECGF fusion protein Ag3052
• Vollständiger Name: thymidine phosphorylase
• Berechnetes Molekulargewicht: 482 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC018160
• Gene symbol: Thymidine phosphorylase
• Gene ID (NCBI): 1890
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Platelet derived endothelial cell growth factor (PD-ECGF), also known as thymidine phosphorylase (TP), induces migration and angiogenesis in endothelial and tumor cells, and was upregulated in various malignancies compared to that in normal tissues. Interestingly, PD-ECGF has dual effect on tumor development and chemotherapy. It could stimulate cancer cell migration and proliferation. On the other hand, some chemotherapeutic agents (5-fluorouracil, capecitabine, etc.) were converted to their active forms through TP enzymes.

Publikationen

Species	Application	Title
human	WB	Oncogene; The proteomic characterization of the peritumor microenvironment in human hepatocellular carcinoma.; Authors - Yuhan Gu
human	FC	Oncotarget; PMID: 29707135; Authors - Toshiki Iwai
mouse	WB,IF	Acta Pharmacol Sin; Exploring the protective effects of schizandrol A in acute myocardial ischemia mice by comprehensive metabolomics profiling integrated with molecular mechanism studies.; Authors - Qiong Lai
mouse	WB	J Ovarian Res; 4-Methylumbelliferone inhibits ovarian cancer growth by suppressing thymidine phosphorylase expression.; Authors - Ryosuke Tamura
human	IHC	Mol Imaging Biol; Evaluation of [(18)F]Fluorothymidine as a Biomarker for Early Therapy Response in a Mouse Model of Colorectal Cancer.; Authors - Sara Rapic
mouse	WB	Antioxidants (Basel); D-Penicillamine Reveals the Amelioration of Seizure-Induced Neuronal Injury via Inhibiting Aqp11-Dependent Ferroptosis; Authors - Nan Yang