Progranulin/PGRN Polyklonaler Antikörper

Progranulin/PGRN Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA, IF

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 10053-1-AP

Synonyme

granulin, Granulin-1, Granulin precursor, Glycoprotein of 88 Kda, Glycoprotein 88

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Galerie der Validierungsdaten

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  WB analysis using 10053-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10053-1-AP (Granulin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10053-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10053-1-AP (Granulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10053-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10053-1-AP (Granulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10053-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10053-1-AP (Granulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10053-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10053-1-AP (Granulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10053-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10053-1-AP (Granulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10053-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10053-1-AP (Granulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

10053-1-AP bindet in WB, IHC, ELISA, IF Progranulin/PGRN und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Progranulin/PGRN fusion protein Ag0010
• Vollständiger Name: granulin
• Berechnetes Molekulargewicht: 64 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC010577
• Gene symbol: Granulin
• Gene ID (NCBI): 2896
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRN, also known as PGRN or PCDGF, is a cysteine-rich protein of 68.5 kDa that is typically secreted into a highly glycosylated 88 kDa form. PGRN is a unique growth factor that plays an important role in cutaneous wound healing. It has an anti-inflammatory effect and promotes cell proliferation. When PCDGF is degraded to several 6-25 kDa fragments, called granulins (GRNs) by neutrophil proteases, a pro-inflammatory reaction occurs. PGRN is widely expressed, particularly in epithelial cells, immune cells, neurons, and chondrocytes. High levels of PGRN expression have been reported in human cancers, and its expression is closely correlated with the development and metastasis of several cancers. The recent discovery that mutations in the gene encoding for pro-granulin (GRN) cause frontotemporal lobar degeneration (FTLD), and other neurodegenerative diseases leading to dementia, has brought renewed interest in progranulin and its functions in the central nervous system. Several in vitro studies have revealed that PGRN is classified into two isoforms according to its glycosylation status, the glycosylated immature isoform (58-68 kDa) and the fully glycosylated mature secretory isoform (∼88 kDa) (PMID: 25838514).

Publikationen

Species	Application	Title
human	IHC	Chin Med J (Engl); Expression of PC cell-derived growth factor and vascular endothelial growth factor in esophageal squamous cell carcinoma and their clinicopathologic significance.; Authors - Chen Xiang-yu XY
	IF	Cell Prolif; m6 A promotes planarian regeneration; Authors - Guanshen Cui

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