CPB1 Polyklonaler Antikörper

CPB1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12600-1-AP

Synonyme

Carboxypeptidase B, carboxypeptidase B1 (tissue), CPB, CPB1, Pancreas specific protein, PASP, PCPB

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Galerie der Validierungsdaten

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  WB analysis of mouse pancreas using 12600-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12600-1-AP (CPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 12600-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12600-1-AP (CPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat pancreas using 12600-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 12600-1-AP (CPB1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 12600-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12600-1-AP (CPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12600-1-AP (CPB1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12600-1-AP (CPB1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 12600-1-AP (CPB1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 12600-1-AP (CPB1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:50.

• 

  IHC staining of human pancreas using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:50.

• 

  IHC staining of human pancreas using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 12600-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Pankreasgewebe, Ratten-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, humanes Nierengewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below

Produktinformation

12600-1-AP bindet in WB, IHC, ELISA CPB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CPB1 fusion protein Ag3243
• Vollständiger Name: carboxypeptidase B1 (tissue)
• Berechnetes Molekulargewicht: 417 aa, 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC015338
• Gene symbol: CPB1
• Gene ID (NCBI): 1360
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PASP(pancreas-specific protein) is also named as CPB1, CPB, PCPB and belongs to the peptidase M14 family. This protein modulates the plasminogen system both in the matrix and on the cell surface, thus leading to the inhibition of endothelial cell movement and tube formation. It is initially described as a pancreatic metallocarboxypeptidase and is a marker for acute pancreatitis(PMID:19717511). The deduced 416-amino acid preproenzyme has a 15-amino acid signal peptide and a 95-amino acid activation peptide.

Publikationen

Species	Application	Title
human	IHC	Cancers (Basel); Is Carboxypeptidase B1 a Prognostic Marker for Ductal Carcinoma In Situ?; Authors - Charu Kothari
human	WB,IHC	Transl Oncol; Label-Free Quantitative Proteomics Unravels Carboxypeptidases as the Novel Biomarker in Pancreatic Ductal Adenocarcinoma.; Authors - Yang Song
rat	WB	Arch Gynecol Obstet; Oviductus Ranae protein hydrolyzate prevents menopausal osteoporosis by regulating TGFβ/BMP2 signaling.; Authors - Xiaohua Li