PARP2 Polyklonaler Antikörper

PARP2 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20555-1-AP

Synonyme

ADPRT2, ADPRT-2, ADPRTL2, ARTD2, hPARP-2, pADPRT-2, PARP2, PARP-2, Poly [ADP-ribose] polymerase 2

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Galerie der Validierungsdaten

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  WB analysis using 20555-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20555-1-AP (PARP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 20555-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20555-1-AP (PARP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20555-1-AP (PARP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20555-1-AP (PARP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20555-1-AP (PARP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20555-1-AP (PARP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 20555-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20555-1-AP (PARP2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of rat brain using 20555-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20555-1-AP (PARP2 antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maushirngewebe, Maushodengewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

20555-1-AP bindet in IHC, ELISA PARP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: poly (ADP-ribose) polymerase 2
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 60-66 kDa
• GenBank-Zugangsnummer: NM_005484
• Gene symbol: PARP2
• Gene ID (NCBI): 10038
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PARP2, also named as ADPRT2 and ADPRTL2, is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PRP2 catalyzes the reaction: NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor. The antibody is specific to PARP2 (C-terminal). PARP2 can be detected as about 62 kDa (PMID: 10364231).