PARK7/DJ-1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PARK7/DJ-1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Rezensionen (2)

Kat-Nr. : 11681-1-AP

Synonyme

PARK7, PARK7,DJ-1, PARK 7, Maillard deglycase, EC:3.1.2.-, EC:3.5.1.-, EC:3.5.1.124

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 11681-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11681-1-AP (PARK7/DJ-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11681-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11681-1-AP (PARK7,DJ-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11681-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11681-1-AP (PARK7,DJ-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11681-1-AP

  IP result of anti-PARK7/DJ-1 (IP:11681-1-AP, 4ug; Detection:11681-1-AP 1:1000) with HeLa cells lysate 1200ug.

• 

  IHC staining of human kidney using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11681-1-AP (PARK7/DJ-1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11681-1-AP (PARK7/DJ-1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11681-1-AP (PARK7/DJ-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11681-1-AP (PARK7/DJ-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 11681-1-AP (PARK7/DJ-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 11681-1-AP (PARK7/DJ-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11681-1-AP (PARK7/DJ-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11681-1-AP (PARK7/DJ-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11681-1-AP (PARK7/DJ-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11681-1-AP (PARK7/DJ-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 11681-1-AP (PARK7/DJ-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11681-1-AP (PARK7/DJ-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11681-1-AP (PARK7/DJ-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11681-1-AP (PARK7/DJ-1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11681-1-AP (PARK7/DJ-1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11681-1-AP (PARK7,DJ-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11681-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11681-1-AP (PARK7,DJ-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 11681-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 11681-1-AP (PARK7/DJ-1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Leberkarzinomgewebe, humanes Nierengewebe, Maushirngewebe, Mausnierengewebe, Rattenhirngewebe, Rattenlebergewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

11681-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PARK7/DJ-1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PARK7/DJ-1 fusion protein Ag2287
• Vollständiger Name: Parkinson disease (autosomal recessive, early onset) 7
• Berechnetes Molekulargewicht: 189 aa, 20 kDa
• Beobachtetes Molekulargewicht: 20 kDa, 25 kDa
• GenBank-Zugangsnummer: BC008188
• Gene symbol: PARK7
• Gene ID (NCBI): 11315
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PARK7, also named as DJ1, belongs to the peptidase C56 family. It protects cells against oxidative stress and cell death. PARK7 plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. It eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. PARK7 has cell-growth promoting activity and transforming activity. It may function as a redox-sensitive chaperone. It's precursor undergoes a cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. The amino acid replace at 166 (L → P) reduces PARK7 protein stability and leads to increased degradation. The predicted MW of this protein is 20 kDa, An additional 25 kDa band can be observed due to modification (PMID: 31767755).

Publikationen

Species	Application	Title
human	WB	Nat Chem Biol; Chemical proteomics reveals new targets of cysteine sulfinic acid reductase.; Authors - Salma Akter
mouse	WB	Nat Commun; Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength.; Authors - Zhisheng Xu ; KD Validated
mouse	IF	PLoS Biol; Extracellular DJ-1 induces sterile inflammation in the ischemic brain.; Authors - Koutarou Nakamura
human	WB	Mol Cell Proteomics; Chemoproteomics Reveals Chemical Diversity and Dynamics of 4-Oxo-2-nonenal Modifications in Cells.; Authors - Rui Sun
human, mouse	WB,IF	Mol Cell Biol; ROS-mediated DJ-1 monomerization modulates intracellular trafficking involving Karyopherin β2.; Authors - Benny Björkblom
human	WB	J Cell Mol Med; FKBP3 aggravates the malignant phenotype of diffuse large B-cell lymphoma by PARK7-mediated activation of Wnt/β-catenin signalling; Authors - Xiaojing Xing

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

X (Verified Customer) (07-11-2022)

A very good and clean antibody with the right molecular weight for WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain lysate

PARK7/DJ-1 Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:11681-1-AP)

FH

Daniel (Verified Customer) (04-16-2019)

Specific band in RCC4 cells

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human kidney cancer cells (RCC4)

PARK7/DJ-1 Antibody Western Blot, validation (1:1000 dilution) in Human kidney cancer cells (RCC4) (Cat no:11681-1-AP)