PANX1 Polyklonaler Antikörper

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• KD/KO Validated

PANX1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, ELISA, IF

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 12595-1-AP

Synonyme

MRS1, pannexin 1, PANX1, PX1, UNQ2529

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Galerie der Validierungsdaten

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  WB analysis using 12595-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12595-1-AP (PANX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 12595-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12595-1-AP (PANX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, C2C12-Zellen, K-562-Zellen, MDA-MB-231-Zellen, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Produktinformation

12595-1-AP bindet in WB, IHC, ELISA, IF PANX1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PANX1 fusion protein Ag3174
• Vollständiger Name: pannexin 1
• Berechnetes Molekulargewicht: 426 aa, 48 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa, 90 kDa
• GenBank-Zugangsnummer: BC016931
• Gene symbol: PANX1
• Gene ID (NCBI): 24145
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PANX1, also named as MRS1, belongs to the pannexin family. It plays a role as a Ca2+-leak channel to regulate ER Ca2+ homeostasis. It is a structural component of the gap junctions and the hemichannels. PANX1 induced the formation of intercellular channels with characteristics of gap junctions. PANX1 is 48-kDa protein which contains 4 transmembrane domains and cytoplasmic N and C termini. It is a mediator of find-me signal/nucleotide release from apoptotic cells. Western blot for Panx1 was also seen in two bands at 45~50 kDa and ~90 kDa. The band of ~90 kDa corresponding to the Panx1 dimer size. (PMID: 24590064, PMID: 19009624)

Publikationen

Species	Application	Title
human	IHC	Theranostics; Mitochondrial Protein UQCRC1 is Oncogenic and a Potential Therapeutic Target for Pancreatic Cancer.; Authors - Qing Wang
human	WB	Sci Signal; Structures of human pannexin-1 in nanodiscs reveal gating mediated by dynamic movement of the N terminus and phospholipids.; Authors - Maki Kuzuya
human	WB	PLoS Pathog; Defining the proteolytic landscape during enterovirus infection.; Authors - Mohsan Saeed
rat	WB	Int J Mol Sci; Age-Dependent Activation of Pannexin1 Function Contributes to the Development of Epileptogenesis in Autosomal Dominant Sleep-related Hypermotor Epilepsy Model Rats; Authors - Kouji Fukuyama
human	IHC	Front Oncol; Construction of a Novel Ferroptosis-Related Gene Signature for Predicting Survival of Patients With Lung Adenocarcinoma.; Authors - Xiaojie Song
mouse	WB	Biochim Biophys Acta Mol Cell Res; TGF-β2 increases cell-cell communication in chondrocytes via p-Smad3 signalling.; Authors - Mengmeng Duan