PANK1 Polyklonaler Antikörper

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• KD/KO Validated

PANK1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 11768-1-AP

Synonyme

hPanK, hPanK1, PANK, PANK1, PANK1a, PANK1b, pantothenate kinase 1, Pantothenic acid kinase 1

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Galerie der Validierungsdaten

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  WB analysis of Raji using 11768-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11768-1-AP (PANK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 11768-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11768-1-AP (PANK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SW 1990 using 11768-1-AP

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 11768-1-AP (PANK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11768-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11768-1-AP (PANK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11768-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11768-1-AP (PANK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Herzgewebe, MCF-7-Zellen, SW 1990-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below

Produktinformation

11768-1-AP bindet in WB, ELISA PANK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PANK1 fusion protein Ag2324
• Vollständiger Name: pantothenate kinase 1
• Berechnetes Molekulargewicht: 598 aa, 64 kDa
• Beobachtetes Molekulargewicht: 61-64 kDa, 40-45 kDa
• GenBank-Zugangsnummer: BC026296
• Gene symbol: PANK1
• Gene ID (NCBI): 53354
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PANK1(Pantothenate kinase 1) is also named as PANk and belongs to the type II pantothenate kinase family. The PANK1 protein, which catalyzes the rate-limiting step of coenzyme A biosynthesis, is also upregulated by p53(PMID:20833636). It also catalyzes the cytosolic phosphorylation of pantothenate (vitamin B5) and derivatives. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 64 kDa, 42 kDa, 36kDa and 45 kDa,respectively.

Publikationen

Species	Application	Title
mouse	WB	J Cell Mol Med; PMID: 32048816; Authors - Lu Yang ; KD Validated
human	WB	Int J Biol Sci; Pantothenate Kinase 1 Inhibits the Progression of Hepatocellular Carcinoma by Negatively Regulating Wnt/β-catenin Signaling.; Authors - Yuyuan Zi ; KD Validated
human	WB	Mol Med Rep; Dioscin inhibits SCC15 cell proliferation via the RASSF1A/MST2/YAP axis.; Authors - Hui Tian