PAK6 Polyklonaler Antikörper

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• KD/KO Validated

PAK6 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 13539-1-AP

Synonyme

p21 activated kinase 6, PAK 6, PAK6

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Galerie der Validierungsdaten

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  WB analysis using 13539-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13539-1-AP (PAK6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 13539-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 13539-1-AP (PAK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 13539-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13539-1-AP (PAK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 13539-1-AP

  IP result of anti-PAK6 (IP:13539-1-AP, 4ug; Detection:13539-1-AP 1:300) with mouse testis tissue lysate 4000 ug.

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  IHC staining of human prostate cancer using 13539-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13539-1-AP (PAK6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 13539-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13539-1-AP (PAK6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 13539-1-AP

  Immunofluorescent analysis of HEK-293 cells using 13539-1-AP (PAK6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Raji-Zellen, Rattenhodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Produktinformation

13539-1-AP bindet in WB, IP, IHC, IF, ELISA PAK6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PAK6 fusion protein Ag4467
• Vollständiger Name: p21 protein (Cdc42/Rac)-activated kinase 6
• Berechnetes Molekulargewicht: 681 aa, 75 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC035596
• Gene symbol: PAK6
• Gene ID (NCBI): 56924
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAK6(p21-activated kinase 6) is a serine/threonine kinase belonging to the p21-activated kinase (PAK) family. .This protein plays a role in the regulation of gene transcription and may protect cells from apoptosis through phosphorylation of BAD. Northern blot analysis revealed that PAK6 is highly expressed in testis and prostate tissues (PMID:11278661). The immunogen of this antibody is the C-terminal of PAK6. This antibody has weak cross-reaction with other PAK members.

Publikationen

Species	Application	Title
human	WB,IHC	Oncol Lett; PAK6 promotes cervical cancer progression through activation of the Wnt/β-catenin signaling pathway.; Authors - Qin Yang ; KD Validated
rat	WB	J Pain Res; Proteomics analysis of the amygdala in rats with CFA-induced pain aversion with electro-acupuncture stimulation.; Authors - Yuanyuan Wu
human	WB, IHC	Mol Med Rep; PMID: 26459798; Authors - Chunhui Liu
human	WB	BMC Nephrol; Urinary exosome proteins PAK6 and EGFR as noninvasive diagnostic biomarkers of diabetic nephropathy; Authors - Tao Li