PAIP1 Polyklonaler Antikörper

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• KD/KO Validated

PAIP1 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 10675-1-AP

Synonyme

Polyadenylate-binding protein-interacting protein 1, Poly(A)-binding protein-interacting protein 1, PAIP-1, PAIP 1, PABP-interacting protein 1

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 10675-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10675-1-AP (PAIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COS-7 using 10675-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 10675-1-AP (PAIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 10675-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10675-1-AP (PAIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 10675-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10675-1-AP (PAIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10675-1-AP

  IP result of anti-PAIP1 (IP:10675-1-AP, 4ug; Detection:10675-1-AP 1:300) with HeLa cells lysate 3000 ug.

• 

  IF Staining of MCF-7 using 10675-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PAIP1 antibody 10675-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, BxPC-3-Zellen, COS-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Produktinformation

10675-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PAIP1 und zeigt Reaktivität mit Affe, human

• Getestete Reaktivität: Affe, human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PAIP1 fusion protein Ag1070
• Vollständiger Name: poly(A) binding protein interacting protein 1
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 60 kDa, 51 kDa, 40 kDa
• GenBank-Zugangsnummer: BC005295
• Gene symbol: PAIP1
• Gene ID (NCBI): 10605
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

In initiation of translation in eukaryotes, binding of the small ribosomal subunit to mRNA requires recognition of the 5-prime cap structure by the cap-binding complex eIF4F. eIF4F consists of eIF4E, eIF4A, and eIF4G. Translation initiation is further regulated by the mRNA 3-prime poly(A) tail and the poly(A)-binding protein (PABC1). PAIP1 interacts with PABC1 and some eIF4 complexes [PMID:9548260]. It is a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs, and associates with EIF4A and PABPC1 may potentiate contacts between mRNA termini [PMID:11051545].

Publikationen

Species	Application	Title
human	WB	Biochem J; Biological Insights into the Expression of Translation Initiation Factors from Recombinant CHOK1SV Cell Lines and their Relationship to Enhanced Productivity.; Authors - Emma Jane Mead
human	WB,IHC,IF	Biol Reprod; Murine PAIP1 stimulates translation of spermiogenic mRNAs stored by YBX2 via its interaction with YBX2.; Authors - Yue He
human	WB,IHC	Hum Pathol; Paip1 predicts poor prognosis and promotes tumor progression through AKT/GSK-3β pathway in lung adenocarcinoma.; Authors - Yixuan Wang ; KD Validated
human	WB,IF	Exp Cell Res; Role of Paip1 on angiogenesis and invasion in pancreatic cancer.; Authors - Hongjian Guan ; KD Validated
human	WB,IHC	Cancer Res Treat; Paip1 Indicated Poor Prognosis in Cervical Cancer and Promoted Cervical Carcinogenesis.; Authors - Nan Li ; KD Validated
human	WB	Arch Biochem Biophys; YAP1-activated ZNF131 promotes hepatocellular carcinoma cell proliferation through transcriptional regulation of PAIP1; Authors - Guozhi Yin