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PABPC1,PABP Polyklonaler Antikörper

PABPC1,PABP Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, RIP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10970-1-AP

Synonyme

PABP, PABP1, PABPC1, PABPC 1, PABP-1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHCT 116-Zellen, A549-Zellen, HeLa-Zellen, HT-29-Zellen, Maushodengewebe, MCF-7-Zellen, NCI-H1299-Zellen, PC-3-Zellen, Rattenhodengewebe
Erfolgreiche IPMaushodengewebe
Erfolgreiche Detektion in IHChumanes Pankreasgewebe, humanes Hodengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCMCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:5000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:20-1:200
Immunfluoreszenz (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

10970-1-AP bindet in WB, IHC, IF/ICC, IP, RIP, ELISA PABPC1,PABP und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PABPC1,PABP fusion protein Ag1422
Vollständiger Name poly(A) binding protein, cytoplasmic 1
Berechnetes Molekulargewicht 71 kDa
Beobachtetes Molekulargewicht 71 kDa
GenBank-ZugangsnummerBC015958
Gene symbol PABPC1
Gene ID (NCBI) 26986
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation [PMID: 21989405]. Polyadenylate-binding protein 1 (PABPC1) is a cytoplasmic-nuclear shuttling protein important for protein translation initiation, and both RNA processing and stability. In the cytoplasm, PABPC1 binds to the 3' poly(A) tail of eukaryotic mRNAs through its RNA-recognition motifs (RRM) and interacts with the N-terminus of eIF4G, part of the eIF4F complex associated with the 5' cap structure [PMID:20009508, 17381337].

Protokolle

Produktspezifische Protokolle
WB protocol for PABPC1,PABP antibody 10970-1-APProtokoll herunterladen
IHC protocol for PABPC1,PABP antibody 10970-1-APProtokoll herunterladen
IF protocol for PABPC1,PABP antibody 10970-1-APProtokoll herunterladen
IP protocol for PABPC1,PABP antibody 10970-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Exp Mol Med

The deubiquitinating enzyme STAMBP is a newly discovered driver of triple-negative breast cancer progression that maintains RAI14 protein stability

Authors - Qianqian Yang
humanWB

Nat Commun

An oncopeptide regulates m6A recognition by the m6A reader IGF2BP1 and tumorigenesis.

Authors - Song Zhu
mouseWB

Dev Cell

DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice

Authors - Dingfeng Zou
humanWB

J Exp Med

E3 ligase MKRN3 is a tumor suppressor regulating PABPC1 ubiquitination in non-small cell lung cancer.

Authors - Ke Li
humanWB

Clin Transl Med

LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing

Authors - Qi Wen Chen
ratIHC

Leukemia

An atlas of bloodstream-accessible bone marrow proteins for site-directed therapy of acute myeloid leukemia.

Authors - L Angenendt

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Elisa (Verified Customer) (01-13-2023)

PABPC1 (Poly(A) Binding Protein Cytoplasmic 1) staining (in magenta) and Hoechst (nuclear staining) in blue. PABPC1 ab shows a clear citoplasma staining. Method: RPE1 cells were fixed in cold methanol for 10' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 10'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-488-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Human retinal pigment epithelial-1 (RPE-1)
PABPC1,PABP Antibody Immunofluorescence validation (1:200 dilution) in Human retinal pigment epithelial-1 (RPE-1) (Cat no:10970-1-AP)
FH

Zee (Verified Customer) (01-28-2020)

It worked very well when I performed western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse heart tissue
FH

George (Verified Customer) (09-11-2019)

Rb PABP Ig shows very little endogenous background staining with clear dispersed staining seen both in the nucleus and cytoplasm. 90mins heat shock at 42oC led to granular formation in the cytoplasm

  • Applications: Immunohistochemistry, Other,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Spinal Cord/Cortical Slice Culture