P4HA2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

P4HA2 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

19

Kat-Nr. : 13759-1-AP

Synonyme

4 PH alpha 2, P4HA2

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Galerie der Validierungsdaten

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  WB analysis using 13759-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13759-1-AP (P4HA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13759-1-AP

  WB result of P4HA2 antibody (13759-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P4HA2 transfected HeLa cells.

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  WB analysis of HeLa using 13759-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13759-1-AP (P4HA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13759-1-AP

  IP result of anti-P4HA2 (IP:13759-1-AP, 4ug; Detection:13759-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IHC staining of human cervical cancer using 13759-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13759-1-AP (P4HA2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 13759-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13759-1-AP (P4HA2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 13759-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13759-1-AP (P4HA2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas cancer using 13759-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13759-1-AP (P4HA2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse ovary tissue using 13759-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 26424-1-AP( LHCGR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary tissue using 13759-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 26424-1-AP( LHCGR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 14 publications below
• IHC: See 7 publications below
• IF: See 3 publications below

Produktinformation

13759-1-AP bindet in WB, IP, IF, IHC, ELISA P4HA2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: P4HA2 fusion protein Ag4714
• Vollständiger Name: prolyl 4-hydroxylase, alpha polypeptide II
• Berechnetes Molekulargewicht: 533 aa, 61 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: BC035813
• Gene symbol: P4HA2
• Gene ID (NCBI): 8974
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Prolyl 4-hydroxylase catalyzes the posttranslational formation of 4-hydroxyproline in collagens. The vertebrate enzyme is a tetramer of 2 alpha subunits and 2 beta subunits. P4HA2 is one of at least 2 alpha subunit isoforms. It catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. This pprotein has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB,IHC,IF	Hepatology; P4HA2 induces hepatic ductular reaction and biliary fibrosis in chronic cholestatic liver diseases; Authors - Jun Zhang ; KD Validated
human	IHC	Blood; Prolyl 4-hydroxylase 2 promotes B-cell lymphoma progression via hydroxylation of Carabin.; Authors - Wei Jiang
		Nat Commun; Melanoblast transcriptome analysis reveals pathways promoting melanoma metastasis.; Authors - Kerrie L Marie
human	WB	EBioMedicine; Aspirin targets P4HA2 through inhibiting NF-κB and LMCD1-AS1/let-7g to inhibit tumour growth and collagen deposition in hepatocellular carcinoma.; Authors - Tianjiao Wang
human	WB	Cancer Res; Epigenetic SMAD3 repression in tumor-associated fibroblasts impairs fibrosis and response to the antifibrotic drug nintedanib in lung squamous cell carcinoma.; Authors - Rafael Ikemori
human	WB,IHC	Oncogene; Hepatitis B virus X protein promotes the development of liver fibrosis and hepatoma through downregulation of miR-30e targeting P4HA2 mRNA.; Authors - G X Feng