• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

P2RX5 Monoklonaler Antikörper

P2RX5 Monoklonal Antikörper für FC, IF, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2A2A6

Kat-Nr. : 66430-1-Ig

Synonyme

ATP receptor, P2RX5, P2X purinoceptor 5, P2X5, P2X5R, Purinergic receptor

Galerie der Validierungsdaten

Filter:
  • Western Blot (WB) analysis of various lysates using P2RX5 Monoclonal antibody (66430-1-Ig)

    WB analysis using 66430-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66430-1-Ig (P2RX5 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using P2RX5 Monoclonal antibody (66430-1-Ig)

    WB analysis of pig brain using 66430-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66430-1-Ig (P2RX5 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse brown adipose tissue using P2RX5 Monoclonal antibody (66430-1-Ig)

    IF Staining of mouse brown adipose using 66430-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brown adipose tissue using P2RX5 antibody (66430-1-Ig, Clone: 2A2A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of Jurkat cells using P2RX5 Monoclonal antibody (66430-1-Ig)

    FC experiment of Jurkat using 66430-1-Ig

    1X10^6 Jurkat cells were stained with 0.20 ug Anti-Human P2RX5 (66430-1-Ig, Clone:2A2A6) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution of 1:1000. Cells were fixed with 90% MeOH.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, Hausschwein-Hirngewebe, Jurkat-Zellen, K-562-Zellen, Maushirngewebe, Rattenhirngewebe
Erfolgreiche Detektion in IFbraunes Mausfettgewebe
Erfolgreiche Detektion in FCJurkat-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:20000
Immunfluoreszenz (IF)IF : 1:200-1:800
Durchflusszytometrie (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66430-1-Ig bindet in WB, IF, FC, ELISA P2RX5 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen P2RX5 fusion protein Ag5297
Vollständiger Name purinergic receptor P2X, ligand-gated ion channel, 5
Berechnetes Molekulargewicht 422 aa, 47 kDa
Beobachtetes Molekulargewicht 52 kDa
GenBank-ZugangsnummerBC039015
Gene symbol P2RX5
Gene ID (NCBI) 5026
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Protokolle

Produktspezifische Protokolle
WB protocol for P2RX5 antibody 66430-1-IgProtokoll herunterladen
IF protocol for P2RX5 antibody 66430-1-IgProtokoll herunterladen
FC protocol for P2RX5 antibody 66430-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen