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OTUD6B Polyklonaler Antikörper

OTUD6B Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25430-1-AP

Synonyme

CGI 77, DUBA 5, DUBA5, OTU domain containing 6B, OTUD6B

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of various lysates using OTUD6B Polyclonal antibody (25430-1-AP)

    WB analysis using 25430-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25430-1-AP (OTUD6B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using OTUD6B Polyclonal antibody (25430-1-AP)

    WB analysis of HeLa using 25430-1-AP

    WB result of OTUD6B antibody (25430-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OTUD6B transfected HeLa cells.

  • Western Blot (WB) analysis of MCF-7 cells using OTUD6B Polyclonal antibody (25430-1-AP)

    WB analysis of MCF-7 using 25430-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25430-1-AP (OTUD6B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using OTUD6B Polyclonal antibody (25430-1-AP)

    WB analysis of Jurkat using 25430-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25430-1-AP (OTUD6B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using OTUD6B Polyclonal antibody (25430-1-AP)

    WB analysis of HEK-293 using 25430-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25430-1-AP (OTUD6B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using OTUD6B Polyclonal antibody (25430-1-AP)

    IHC staining of human ovary tumor using 25430-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25430-1-AP (OTUD6B Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using OTUD6B Polyclonal antibody (25430-1-AP)

    IHC staining of human ovary tumor using 25430-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25430-1-AP (OTUD6B Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using OTUD6B Polyclonal antibody (25430-1-AP)

    IF Staining of PC-3 using 25430-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 25430-1-AP (OTUD6B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen, PC-3-Zellen
Erfolgreiche Detektion in IHChumanes Ovarialkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFPC-3-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
Immunhistochemie (IHC)IHC : 1:20-1:200
Immunfluoreszenz (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

25430-1-AP bindet in WB, IHC, IF, ELISA OTUD6B und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen OTUD6B fusion protein Ag22044
Vollständiger Name OTU domain containing 6B
Berechnetes Molekulargewicht 293 aa, 34 kDa
Beobachtetes Molekulargewicht 34-40 kDa
GenBank-ZugangsnummerBC029760
Gene symbol OTUD6B
Gene ID (NCBI) 51633
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Protokolle

Produktspezifische Protokolle
WB protocol for OTUD6B antibody 25430-1-APProtokoll herunterladen
IHC protocol for OTUD6B antibody 25430-1-APProtokoll herunterladen
IF protocol for OTUD6B antibody 25430-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Dorota (Verified Customer) (04-11-2019)

Antibody works perfectly for western blotting, however I could not optimise it for immunofluorescence. I tried series of dilutions and couple of different blocking/fixing methods, but there was no difference between knockdown and control conditions in any of them.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:500-1:5000
  • Cell Tissue Type: BT549
OTUD6B Antibody Western Blot,Immunofluorescence, validation (1:500-1:5000 dilution) in BT549 (Cat no:25430-1-AP)