OSBPL9 Polyklonaler Antikörper

OSBPL9 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11879-1-AP

Synonyme

ORP 9, ORP9, OSBP related protein 9, OSBP4, OSBPL9

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Galerie der Validierungsdaten

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  WB analysis of human brain using 11879-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11879-1-AP (OSBPL9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11879-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11879-1-AP (OSBPL9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11879-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11879-1-AP (OSBPL9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 11879-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11879-1-AP (OSBPL9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 11879-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11879-1-AP (OSBPL9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 11879-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11879-1-AP (OSBPL9 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 11879-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11879-1-AP (OSBPL9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 11879-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11879-1-AP (OSBPL9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 11879-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11879-1-AP (OSBPL9 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, humanes Hodengewebe, Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

11879-1-AP bindet in WB, IHC, ELISA OSBPL9 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OSBPL9 fusion protein Ag2476
• Vollständiger Name: oxysterol binding protein-like 9
• Berechnetes Molekulargewicht: 736 aa, 83 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC025978
• Gene symbol: OSBPL9
• Gene ID (NCBI): 114883
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ORP9 is expressed as a full-length (ORP9L) or truncated version missing the PH domain (ORP9S).

Publikationen

Species	Application	Title
human	WB	Nature; A phosphoinositide signalling pathway mediates rapid lysosomal repair; Authors - Jay Xiaojun Tan
human	WB	Cell Mol Life Sci; ORP9 and ORP10 form a heterocomplex to transfer phosphatidylinositol 4-phosphate at ER-TGN contact sites; Authors - Ruyue He
human	WB	J Cell Biol; Phosphatidylserine regulates plasma membrane repair through tetraspanin-enriched macrodomains; Authors - Yang E Li ; KO Validated
human		Elife; The ORP9-ORP11 dimer promotes sphingomyelin synthesis; Authors - Birol Cabukusta