OSBP Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

OSBP Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Rezensionen (1)

Kat-Nr. : 11096-1-AP

Synonyme

Oxysterol-binding protein 1, Oxysterol binding protein 1, oxysterol binding protein, OSBP1

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Galerie der Validierungsdaten

• 

  WB analysis using 11096-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11096-1-AP (OSBP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 11096-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11096-1-AP (OSBP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 11096-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 11096-1-AP (OSBP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa cells using 11096-1-AP

  IP result of anti-OSBP (11096-1-AP for IP and Detection).

• 

  IHC staining of human ovary tumor using 11096-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11096-1-AP (OSBP antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 11096-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11096-1-AP (OSBP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Maushirngewebe, Mausnierengewebe, Rattenhirngewebe, Rattennierengewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 15 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

11096-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA OSBP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hefe, Kaninchen, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OSBP fusion protein Ag1578
• Vollständiger Name: oxysterol binding protein
• Berechnetes Molekulargewicht: 89 kDa
• Beobachtetes Molekulargewicht: 89 kDa
• GenBank-Zugangsnummer: BC011581
• Gene symbol: OSBP
• Gene ID (NCBI): 5007
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IF	Nature; A phosphoinositide signalling pathway mediates rapid lysosomal repair; Authors - Jay Xiaojun Tan
human	WB,IF	Gastroenterology; Oxysterol-Binding Protein Is a Phosphatidylinositol 4-Kinase Effector Required for HCV Replication Membrane Integrity and Cholesterol Trafficking.; Authors - Hongliang Wang
human	WB	Cell Host Microbe; The antiviral effector IFITM3 disrupts intracellular cholesterol homeostasis to block viral entry.; Authors - Samad Amini-Bavil-Olyaee
human	WB,IF	PLoS Pathog; Modulation of the Host Lipid Landscape to Promote RNA Virus Replication: The Picornavirus Encephalomyocarditis Virus Converges on the Pathway Used by Hepatitis C Virus.; Authors - Cristina M Dorobantu ; KD Validated
		Int J Mol Sci; A Small Molecule, 4-Phenylbutyric Acid, Suppresses HCV Replication via Epigenetically Induced Hepatic Hepcidin.; Authors - Kiyoon Kim
yeast	WB	Hum Mol Genet; Molecular partners of hNOT/ALG3, the human counterpart of the Drosophila NOT and yeast ALG3 gene, suggest its involvement in distinct cellular processes relevant to congenital disorders of glycosylation, cancer, neurodegeneration and a variety of further pathologies.; Authors - Benedikt Hacker

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (09-12-2019)

This antibody could detect endogenous OSBP protein both in WB and IF.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: MEF