ODC1 Monoklonaler Antikörper

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• KD/KO Validated

ODC1 Monoklonal Antikörper für WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB,ELISA

Konjugation

Unkonjugiert

CloneNo.

2A1E3

Kat-Nr. : 67336-1-Ig

Synonyme

ODC, ODC1, Ornithine decarboxylase, ornithine decarboxylase 1

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 67336-1-Ig

  WB result of ODC1 antibody (67336-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ODC1 transfected MCF-7 cells.

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  WB analysis of MCF-7 using 67336-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67336-1-Ig (ODC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 67336-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67336-1-Ig (ODC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HUVEC using 67336-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 67336-1-Ig (ODC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, humanes Plazenta-Gewebe, HUVEC-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67336-1-Ig bindet in WB,ELISA ODC1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ODC1 fusion protein Ag10927
• Vollständiger Name: ornithine decarboxylase 1
• Berechnetes Molekulargewicht: 461 aa, 51 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC025296
• Gene symbol: ODC1
• Gene ID (NCBI): 4953
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ornithine decarboxylase (ODC) is also named as ODC1 and belongs to the Orn/Lys/Arg decarboxylase class-II family. It catalyzes the conversion of ornithine to putrescine, the first step and a major site of regulation of polyamine biosynthesis. The level of ODC is known to be controlled at several sites, namely transcription, translation, and enzyme degradation. Polyamines can stimulate the degradation of ODC as a type of negative feedback control (PMID:8486633). This protein can be phosphorylated in vivo (PMID:8798774). ODC1 can form a homodimer and only the dimer is catalytically active, as the active sites are constructed of residues from both monomers (PMID: 10623504). The molecular mass of ODC1 is 51 kDa, and the homodimer is 106 kDa.