NeutraKine® TGF beta 1 Monoklonaler Antikörper

NeutraKine® TGF beta 1 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

PE Fluorescent Dye

CloneNo.

1E3E9

Kat-Nr. : PE-69012

Synonyme

CED, DPD1, LAP, TGF ?1, TGF beta 1, TGFB, TGFB1, TGFbeta

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Galerie der Validierungsdaten

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  FC experiment of HEK-293 using PE-69012

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug PE Anti-Human NeutraKine® TGF beta 1 (PE-69012, Clone:1E3E9) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: HEK-293-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
Sample-dependent, check data in validation data gallery

Produktinformation

PE-69012 bindet in FC (Intra) NeutraKine® TGF beta 1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NeutraKine® TGF beta 1 fusion protein HZ-1011
• Vollständiger Name: transforming growth factor, beta 1
• Gene symbol: TGFB1
• Gene ID (NCBI): 7040
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.1% sodium azide and 0.5% BSA.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. It is highly expressed in bone. Mutation of TGFB are the cause of Camurati-Engelmann disease (CED) which known as progressive diaphyseal dysplasia 1 (DPD1).

This antibody can be used to neutralize the bioactivity of TGF beta 1.