NeutraKine® SCF Monoklonaler Antikörper

NeutraKine® SCF Monoklonal Antikörper für Neutralization, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

Neutralization, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A10G10

Kat-Nr. : 69030-1-Ig

Synonyme

c Kit ligand, DKFZp686F2250, KIT ligand, Kitl, KITLG, KL 1, Mast cell growth factor, MGF, SCF, SF, SHEP7, Stem cell factor

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Galerie der Validierungsdaten

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  Neutralization experiment of NeutraKine® SCF using 69030-1-Ig

  Recombinant human SCF (Cat.NO. HZ-1024) stimulates proliferation of the MO7e human megakaryoblastic leukemia cell line in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human SCF (30 ng/mL HZ-1024) is neutralized by mouse anti-human SCF monoclonal antibody 69030-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 0.2-1.0 μg/mL

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  ELISA experiment of Recombinant protein using 69030-1-Ig

  Indirect ELISA was carried out by coating recombinant Human SCF (Cat.NO. HZ-1024) at 70 ng/well followed by blocking and adding serial diluted SCF antibody 69030-1-Ig and 69530-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

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Neutralization

Produktinformation

69030-1-Ig bindet in Neutralization, ELISA NeutraKine® SCF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: human Humankine SCF protein HZ-1024
• Vollständiger Name: KIT ligand
• Gene symbol: KITLG
• Gene ID (NCBI): 4254
• Konjugation: Unkonjugiert
• Form: Lyophilized Powder
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: Sterile PBS.
• Endotoxin: <0.1 EU/μg
• Wiederherstellung: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Hintergrundinformationen

Stem cell factor (SCF) also known as mast cell growth factor, steel factor, and kit ligand, is a hematopoietic growth factor. SCF is a critical protein with key roles in the cell such as hematopoiesis, gametogenesis and melanogenesis. SCF activates multiple signal pathways by binding with c-kit receptor. SCF and its receptor, c-kit ligand (KL), are up-regulated in particular human malignancies including gastrointestinal stromal tumor (GISTs), breast cancer, hematopoietic cell, myeloid leukaemia, and glioma. The SCF/c-KIT system also plays a relevant role in cell fate decision, and its activity is widely known to control cell proliferation, differentiation, survival and apoptosis.

This antibody is used to neutralize the bioactivity of SCF.