NeutraKine®FGF basic Monoklonaler Antikörper

NeutraKine®FGF basic Monoklonal Antikörper für ELISA, Neutralization

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

Neutralization, ELISA, Cell treatment

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

3C9C3

Kat-Nr. : 69024-1-Ig

Synonyme

Basic fibroblast growth factor, BFGF, FGF basic, FGF2, FGFB, FGFbasic, HBGF 2

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  Neutralization experiment of NeutraKine®FGF basic using 69024-1-Ig

  Recombinant human FGF Basic (Cat.NO. HZ-1285) stimulates the proliferation of BALB/3T3 cells (mouse fibroblast cell) at low FBS condition in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human FGF Basic (0.1 ng/mL HZ-1285) is neutralized by mouse anti-human FGF Basic monoclonal antibody 69024-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 1-10 μg/mL. Mouse IgG1 Isotype Control monoclonal antibody 66360-1-Ig was served as control (green curve, refer to top X-right Y axis).

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Neutralization

Produktinformation

69024-1-Ig bindet in Neutralization, ELISA, Cell treatment NeutraKine®FGF basic und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: human HumankineFGF basic protein HZ-1285
• Vollständiger Name: fibroblast growth factor 2 (basic)
• Gene symbol: FGF2
• Gene ID (NCBI): 2247
• Konjugation: Unkonjugiert
• Form: Lyophilized Powder
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: Sterile PBS
• Endotoxin: <0.1 EU/μg
• Wiederherstellung: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Hintergrundinformationen

Fibroblast growth factor-2 (FGF-2), also referred to as basic FGF, belongs to a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in at least five different isoforms with distinct properties.

This antibody is used to neutralize the bioactivity of FGFbasic-TS.

Publikationen

Species	Application	Title
human	Cell treatment	Int Immunopharmacol; Cancer-associated fibroblasts promote gastric cancer cell proliferation by paracrine FGF2-driven ribosome biogenesis; Authors - Dandan Li