Nectin-2/CD112 Rekombinanter Antikörper

Nectin-2/CD112 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

CloneNo.

241798A11

Kat-Nr. : FITC-98231

Synonyme

241798A11, CD112, Herpes virus entry mediator B, HveB, mHveB

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  FC experiment of NIH/3T3 using FITC-98231

  1x10^6 NIH/3T3 cells were surface stained with 5 ul FITC Plus Anti-Mouse Nectin-2/CD112 Rabbit RecAb (FITC-98231, Clone:241798A11) or 5 ul FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9). Cells were not fixed.

• 

  FC experiment of NIH/3T3 using FITC-98231

  1x10^6 NIH/3T3 cells were surface stained with 0.25 ug FITC Plus Anti-Mouse Nectin-2/CD112 Rabbit RecAb (FITC-98231, Clone: 241798A11) (red) or 0.25 ug FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9) (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: NIH/3T3-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-98231 bindet in FC Nectin-2/CD112 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Nectin-2/CD112 fusion protein Eg2019
• Vollständiger Name: poliovirus receptor-related 2
• Berechnetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: NM_008990.3
• Gene symbol: Nectin-2/CD112
• Gene ID (NCBI): 19294
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Nectin 2, also named as PVRL2, CD112, HVEB, PRR2 and PVRR2, is an adhesion molecule widely expressed in cell lines of different lineages, including hematopoietic, neuronal, endothelial and epithelial cells. CD112 belongs to a new family of immunoglobulin-like molecules that includes four members (CD111, CD112, PRR3 and CD155) sharing an ectodomain made of three Ig domains, of V and C types. CD112 is expressed in the myelo-monocytic and megakaryocytic hematopoietic lineages and the function in hematopoiesis is currently unknown. CD112 is an intercellular homophilic adhesion. CD112 localizes specifically at adherents junctions via its cytoplasmic interaction with the scaffold F-actin binding protein afadin. Disruption of the murine CD112 gene leads to infertility of male mice with morphologically aberrant spermatozoa. CD112 mediates entry of some alphaherpesvirus mutants (also named HveB) via its V domain. CD112 is involved in cell to cell spreading of viruses.