TXNL6 Polyklonaler Antikörper

TXNL6 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 11203-1-AP

Synonyme

nucleoredoxin like 1, Nucleoredoxin like protein 1, NXNL1, RDCVF, Thioredoxin like protein 6, TXNL6

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 11203-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11203-1-AP (TXNL6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11203-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11203-1-AP (TXNL6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11203-1-AP

  IP result of anti-TXNL6 (IP:11203-1-AP, 4ug; Detection:11203-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human breast cancer using 11203-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11203-1-AP (TXNL6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 11203-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 11203-1-AP (TXNL6 Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 11203-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 11203-1-AP (TXNL6 Antibody) at dilution of 1:50.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 2 publications below

Produktinformation

11203-1-AP bindet in WB, IP, IF, IHC, ELISA TXNL6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TXNL6 fusion protein Ag1691
• Vollständiger Name: nucleoredoxin-like 1
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 26-28 kDa
• GenBank-Zugangsnummer: BC014127
• Gene symbol: NXNL1
• Gene ID (NCBI): 115861
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TXNL6, also named as NXNL1, belongs to the nucleoredoxin family. It may play a role in cone cell viability, slowing down cone degeneration, does not seem to play a role in degenerating rods. TXNL6 is a Novel Oxidative Stress-Induced Reducing System for Methionine Sulfoxide Reductase A Repair of a-Crystallin and Cytochrome C in the Eye Lens. It serve as a reducing system for MsrA repair of the essential lens chaperone a-crystallin/sHSP and mitochondrial cytochrome c. TXNL6 is induced at high levels in human lens epithelial cells exposed to H2O2-induced oxidative stress.(PMID:21079812)

Publikationen

Species	Application	Title
pig	IF	Cell Rep; Two-Step Reactivation of Dormant Cones in Retinitis Pigmentosa.; Authors - Wei Wang
human	WB,IF	PLoS One; TXNL6 is a novel oxidative stress-induced reducing system for methionine sulfoxide reductase a repair of α-crystallin and cytochrome C in the eye lens.; Authors - Brennan Lisa A LA