NXF1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NXF1 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 10328-1-AP

Synonyme

Tip-associated protein, Tip associating protein, Tip associated protein, TAP, mRNA export factor TAP

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 10328-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10328-1-AP (NXF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10328-1-AP

  WB result of NXF1 antibody (10328-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NXF1 transfected HeLa cells.

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  WB analysis using 10328-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10328-1-AP (NXF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10328-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10328-1-AP (NXF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10328-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10328-1-AP (NXF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10328-1-AP

  IP result of anti-NXF1 (IP:10328-1-AP, 4ug; Detection:10328-1-AP 1:500) with HeLa cells lysate 3000 ug.

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  IF Staining of HepG2 using 10328-1-AP

  Immunofluorescent analysis of HepG2 cells using 10328-1-AP (NXF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293T-Zellen, HepG2-Zellen, K-562-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

10328-1-AP bindet in WB, IF/ICC, IP, CoIP, ELISA NXF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Affe, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NXF1 fusion protein Ag0413
• Vollständiger Name: nuclear RNA export factor 1
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 66-70 kDa
• GenBank-Zugangsnummer: BC004904
• Gene symbol: NXF1
• Gene ID (NCBI): 10482
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear RNA export factor 1 (NXF1, synonyms: TAP, MEX67) is one member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1(NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. This protein shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. NXF1 overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. There are some phosphorylation modification sites center on the N-terminal of NXF1.

Publikationen

Species	Application	Title
human	WB	J Cell Biol; Inhibition of pyrimidine synthesis reverses viral virulence factor-mediated block of mRNA nuclear export.; Authors - Zhang Liang L ; KD Validated
		J Cell Biol; Intronless mRNAs transit through nuclear speckles to gain export competence.; Authors - Ke Wang
human	WB	Nucleic Acids Res; Depletion of REF/Aly alters gene expression and reduces RNA polymerase II occupancy.; Authors - Sarah H Stubbs
human	WB	PLoS Genet; Nucleoporin TPR promotes tRNA nuclear export and protein synthesis in lung cancer cells.; Authors - Miao Chen ; KD Validated
monkey	WB,CoIP	Mol Cell Biol; Fragile X mental retardation protein FMRP binds mRNAs in the nucleus.; Authors - Kim Miri M ; KD Validated
human	WB,IF,IP	Chromosoma; XIST RNA exhibits nuclear retention and exhibits reduced association with the export factor TAP/NXF1.; Authors - Cohen Hannah R HR