NUP98-NUP96 Polyklonaler Antikörper

NUP98-NUP96 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12329-1-AP

Synonyme

NUP98 NUP96, Nuclear pore complex protein Nup98-Nup96, Nuclear pore complex protein Nup98, Nuclear pore complex protein Nup96, EC:3.4.21.-

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Galerie der Validierungsdaten

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  WB analysis using 12329-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12329-1-AP (NUP98-NUP96 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12329-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12329-1-AP (NUP98-NUP96 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12329-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12329-1-AP (NUP98-NUP96 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12329-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12329-1-AP (NUP98-NUP96 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 12329-1-AP

  IP result of anti-NUP98-NUP96 (IP:12329-1-AP, 4ug; Detection:12329-1-AP 1:1000) with COLO 320 cells lysate 2000ug.

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  IHC staining of human breast cancer using 12329-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12329-1-AP (NUP98-NUP96 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12329-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12329-1-AP (NUP98-NUP96 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12329-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12329-1-AP (NUP98-NUP96 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12329-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12329-1-AP (NUP98-NUP96 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 12329-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NUP98-NUP96 antibody (12329-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen
• Erfolgreiche IP: COLO 320-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 2 publications below

Produktinformation

12329-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA NUP98-NUP96 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NUP98-NUP96 fusion protein Ag3016
• Vollständiger Name: nucleoporin 98kDa
• Berechnetes Molekulargewicht: 98 kDa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: BC012906
• Gene symbol: NUP98
• Gene ID (NCBI): 4928
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nup98-Nup96 (also known as Nup196, ADIR2 or ADAR2), encoded by NUP98 gene, is a component of the nuclear pore complex (NPC) . It is first synthesized as 186-198 kDa Nup98-Nup96 precursors and the precursors shortly undergo autoproteolysis to give rise to two nucleoporins, the amino-terminal Nup98 (Nup98-N) and carboxy-terminal Nup96 (Nup96-C). Both of Nup98 and Nup96 are localized to the nucleoplasmic side of the NPC. This antibody was raised against the carboxy-terminal region of Nup98-Nup96, it recognizes all Nup98-Nup96 proteins except Nup98-N. Since the autoproteolysis is very rapid this antibody usually detected 98-115 kDa Nup96 protein in multiple cell lines. It is notable that RNA-editing deaminase 1 (Gene ID: 104), also named ADAR2, is a different protein from Nup98-Nup96 (ADAR2).

Publikationen

Species	Application	Title
mouse	WB	Neuron; Nucleoporin Seh1 Interacts with Olig2/Brd7 to Promote Oligodendrocyte Differentiation and Myelination.; Authors - Zhixiong Liu
human	IF	Cell Rep; Antiviral Immune Response as a Trigger of FUS Proteinopathy in Amyotrophic Lateral Sclerosis.; Authors - Tatyana A Shelkovnikova
human	IF	Nat Commun; iU-ExM: nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy; Authors - Vincent Louvel