NUP133 Polyklonaler Antikörper

NUP133 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 12405-1-AP

Synonyme

133 kDa nucleoporin, hNUP133, nucleoporin 133kDa, Nucleoporin Nup133, NUP133

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 12405-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12405-1-AP (NUP133 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12405-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12405-1-AP (NUP133 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12405-1-AP

  IP result of anti-NUP133 (IP:12405-1-AP, 4ug; Detection:12405-1-AP 1:800) with HeLa cells lysate 880ug.

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  IHC staining of human colon cancer using 12405-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12405-1-AP (NUP133 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

12405-1-AP bindet in WB, IP, IHC, ChIP, ELISA NUP133 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NUP133 fusion protein Ag3100
• Vollständiger Name: nucleoporin 133kDa
• Berechnetes Molekulargewicht: 1156 aa, 129 kDa
• Beobachtetes Molekulargewicht: 129 kDa
• GenBank-Zugangsnummer: BC020107
• Gene symbol: NUP133
• Gene ID (NCBI): 55746
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear pore complex protein Nup133 is a protein that in humans is encoded by the NUP133 gene. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells.

Publikationen

Species	Application	Title
rat	WB	Int J Mol Sci; Fetal Zone Steroids and Estrogen Show Sex Specific Effects on Oligodendrocyte Precursor Cells in Response to Oxidative Damage.; Authors - Donna Elizabeth Sunny
human	ChIP	Mol Cell Pediatr; Nup133 and ERα mediate the differential effects of hyperoxia-induced damage in male and female OPCs.; Authors - Donna Elizabeth Sunny