NUMBL Polyklonaler Antikörper

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• KD/KO Validated

NUMBL Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 10111-1-AP

Synonyme

Numb-related protein, Numb-like protein, NUMBLIKE

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 10111-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10111-1-AP (NUMBL antibody at dilution of 1:2000 incubated at 4 degree celsius over night.

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  WB analysis of HeLa using 10111-1-AP

  WB result of NUMBL antibody (10111-1-AP, 1:3000) with si-Control and si-NUMBL transfected HeLa cells.

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  WB analysis of mouse spleen using 10111-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10111-1-AP (NUMBL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 10111-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10111-1-AP (NUMBL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10111-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10111-1-AP (NUMBL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10111-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10111-1-AP (NUMBL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10111-1-AP

  IP result of anti-NUMBL (IP:10111-1-AP, 4ug; Detection:10111-1-AP 1:1000) with HeLa cells lysate 2800 ug.

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  IHC staining of human brain using 10111-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10111-1-AP (NUMBL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 10111-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10111-1-AP (NUMBL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 10111-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10111-1-AP (NUMBL antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 10111-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10111-1-AP (NUMBL antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse cerebellum using 10111-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NUMBL antibody (10111-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-12 using 10111-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using 10111-1-AP (NUMBL antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10111-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10111-1-AP (NUMBL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, Mauslebergewebe, Mauslungengewebe, Mausmilzgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Cerebellum-Gewebe
• Erfolgreiche Detektion in IF/ICC: PC-12-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 3 publications below

Produktinformation

10111-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, ELISA NUMBL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NUMBL fusion protein Ag0156
• Vollständiger Name: numb homolog (Drosophila)-like
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC001794
• Gene symbol: NUMBL
• Gene ID (NCBI): 9253
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Numbl (also known as numblike or Nbl), as a conserved homolog of Drosophila Numb, is specifically expressed in the brain. Numbl is a cytoplasm protein and has redundant functions in embryonic neurogenesis. It plays an important role in ependymal wall integrity and subventricular zone neuroblast survival. However, its expression and function in the central nervous system lesion are still unclear.

Publikationen

Species	Application	Title
mouse	WB	Nat Neurosci; Numb and Numbl are required for maintenance of cadherin-based adhesion and polarity of neural progenitors.; Authors - Rasin Mladen-Roko MR
human	WB,IHC	Cancer Res; Regulation of lung cancer metastasis by Klf4-Numb-like signaling.; Authors - Vaira Valentina V ; KD Validated
human	WB,IHC	J Clin Endocrinol Metab; PMID: 16705073; Authors - Sinha-Hikim Indrani I
mouse	WB	Development; Cleaved Delta like 1 intracellular domain regulates neural development via Notch signal-dependent and -independent pathways.; Authors - Yusuke Okubo
mouse	IHC	Mol Endocrinol; Numb deletion in POMC-expressing cells impairs pituitary intermediate lobe cell adhesion, progenitor cell localization, and neuro-intermediate lobe boundary formation.; Authors - Moran Tyler B TB