NUDT2 Polyklonaler Antikörper

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• KD/KO Validated

NUDT2 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 10484-1-AP

Synonyme

Ap4A hydrolase, Ap4Aase, APAH1, Diadenosine tetraphosphatase, Nudix motif 2, NUDT2

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Galerie der Validierungsdaten

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  WB analysis using 10484-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10484-1-AP (NUDT2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10484-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10484-1-AP (NUDT2 antibody) at dilution of 1:4000 incubated at 4 degree celsius over night.

• 

  WB analysis using 10484-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10484-1-AP (NUDT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10484-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10484-1-AP (NUDT2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 10484-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10484-1-AP (NUDT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10484-1-AP

  IP result of anti-NUDT2 (IP:10484-1-AP, 4ug; Detection:10484-1-AP 1:1000) with HEK-293 cells lysate 2600 ug.

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  IHC staining of human breast cancer using 10484-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10484-1-AP (NUDT2 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10484-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10484-1-AP (NUDT2 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10484-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10484-1-AP (NUDT2 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10484-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10484-1-AP (NUDT2 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Maushirngewebe, Maus-Kolongewebe, Mauslebergewebe, Mausmilzgewebe, Rattenlebergewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

10484-1-AP bindet in WB, IHC, IP, ELISA NUDT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NUDT2 fusion protein Ag0743
• Vollständiger Name: nudix (nucleoside diphosphate linked moiety X)-type motif 2
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC004926
• Gene symbol: NUDT2
• Gene ID (NCBI): 318
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Int J Mol Sci; Role of Nudt2 in Anchorage-Independent Growth and Cell Migration of Human Melanoma; Authors - Sana' Hidmi ; KD Validated
human	IHC	Front Oncol; The functional role of Nudt2 in human triple negative breast cancer; Authors - Rasha Abu-Rahmah