NTHL1 Polyklonaler Antikörper

NTHL1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 11154-1-AP

Synonyme

NTH1, NTHL1, OCTS3

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Galerie der Validierungsdaten

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  WB analysis of human brain using 11154-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11154-1-AP (NTHL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11154-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11154-1-AP (NTHL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11154-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11154-1-AP (NTHL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, humanes Hirngewebe, K-562-Zellen, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IP: See 1 publications below

Produktinformation

11154-1-AP bindet in WB, IP, ELISA NTHL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NTHL1 fusion protein Ag1631
• Vollständiger Name: nth endonuclease III-like 1 (E. coli)
• Berechnetes Molekulargewicht: 34 aa, 3 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC003014
• Gene symbol: NTHL1
• Gene ID (NCBI): 4913
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NTHL1, also named as Endonuclease III-like protein 1 or OCTS3, is a 312 amino acid protein, which belongs to the Nth/MutY family. NTHL1 localizes in the nucleus and is widely expressed with highest levels in heart and lowest levels in lung and liver. NTHL1 has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity and incises damaged DNA at cytosines, thymines and guanines. NTHL1 acts on a damaged strand, 5' from the damaged site and is required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.

Publikationen

Species	Application	Title
rat	WB	FASEB J; Chronic gestational hypoxia accelerates ovarian aging and lowers ovarian reserve in next-generation adult rats.; Authors - Catherine E Aiken
human	WB	Respir Res; Protection of pulmonary epithelial cells from oxidative stress by hMYH adenine glycosylase.; Authors - Kremer Ted M TM
human	WB,IP	NAR Cancer; The DNA repair function of BCL11A suppresses senescence and promotes continued proliferation of triple-negative breast cancer cells; Authors - Elise Vickridge
human	WB	Nucleic Acids Res; The function of BCL11B in base excision repair contributes to its dual role as an oncogene and a haplo-insufficient tumor suppressor gene; Authors - Elise Vickridge

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

James (Verified Customer) (02-17-2023)

Recognises NTHL1 at 34kDa, but has non-specific bands which decrease with siRNA knock-down of NTHL1 at higher molecular weights (150kDa).

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U2OS

NTHL1 Antibody Western Blot validation (1:1000 dilution) in U2OS (Cat no:11154-1-AP)