NT5E,CD73 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

NT5E,CD73 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1G5G8

Kat-Nr. : 67789-1-Ig

Synonyme

5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), CD73, E5NT, Ecto 5 nucleotidase, eN, eNT, NT, NT5, NT5E, NT5E,CD73, NTE

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 67789-1-Ig

  WB result of NT5E,CD73 antibody (67789-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NT5E,CD73 transfected HepG2 cells.

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  WB analysis of A549 using 67789-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67789-1-Ig (NT5E,CD73 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-29 using 67789-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67789-1-Ig (NT5E,CD73 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-87 MG using 67789-1-Ig

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 67789-1-Ig (NT5E,CD73 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 67789-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67789-1-Ig (NT5E,CD73 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 67789-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67789-1-Ig (NT5E,CD73 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 67789-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67789-1-Ig (NT5E,CD73 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 67789-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67789-1-Ig (NT5E,CD73 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat liver using 67789-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using NT5E,CD73 antibody (67789-1-Ig, Clone: 1G5G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of rat liver using 67789-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using NT5E,CD73 antibody (67789-1-Ig, Clone: 1G5G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 67789-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using NT5E,CD73 antibody (67789-1-Ig, Clone: 1G5G8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HepG2-Zellen, HT-29-Zellen, U-87 MG-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe, Rattenlebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67789-1-Ig bindet in WB, IHC, IF, ELISA NT5E,CD73 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NT5E,CD73 fusion protein Ag30616
• Vollständiger Name: 5'-nucleotidase, ecto (CD73)
• Berechnetes Molekulargewicht: 29 kDa, 63 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC015940
• Gene symbol: NT5E
• Gene ID (NCBI): 4907
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).