NSFL1C Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NSFL1C Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 15620-1-AP

Synonyme

NSFL1 (p97) cofactor (p47), NSFL1 cofactor p47, NSFL1C, p47, p97 cofactor p47, UBX1, UBXD10, UBXN2C

---

Galerie der Validierungsdaten

• 

  WB analysis of A431 using 15620-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15620-1-AP (NSFL1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15620-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15620-1-AP (NSFL1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15620-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15620-1-AP (NSFL1C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15620-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15620-1-AP (NSFL1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 15620-1-AP

  IP Result of anti-NSFL1C (IP:15620-1-AP, 4ug; Detection:15620-1-AP 1:1000) with HEK-293 cells lysate 2640ug.

• 

  IHC staining of human lung cancer using 15620-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15620-1-AP (NSFL1C Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 15620-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15620-1-AP (NSFL1C Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 15620-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15620-1-AP (NSFL1C Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 15620-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15620-1-AP (NSFL1C Antibody) at dilution of 1:50 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, Mauslebergewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Produktinformation

15620-1-AP bindet in WB, IP, IHC, IF, ELISA NSFL1C und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NSFL1C fusion protein Ag8040
• Vollständiger Name: NSFL1 (p97) cofactor (p47)
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 47 kDa, 41 kDa
• GenBank-Zugangsnummer: BC002801
• Gene symbol: NSFL1C
• Gene ID (NCBI): 55968
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; Altered cofactor regulation with disease-associated p97/VCP mutations.; Authors - Xiaoyi Zhang
human	WB	Cell Chem Biol; Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase.; Authors - Xiaojun Wu
human	WB	J Biol Chem; Pathogenic mutations in the Valosin-containing protein/p97(VCP) N-domain inhibit the SUMOylation of VCP and lead to impaired stress response.; Authors - Tao Wang
human	WB,IF	J Cell Sci; The p97-UBXN1 complex regulates aggresome formation.; Authors - Sirisha Mukkavalli ; KD Validated
mouse	WB	Evid Based Complement Alternat Med; Shenxian-Shengmai Oral Liquid Improves Sinoatrial Node Dysfunction through the PKC/NOX-2 Signaling Pathway.; Authors - Heng Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Julia (Verified Customer) (07-02-2019)

Clean and specific bands on Western blot at correct MW.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human