NSE Monoklonaler Antikörper

NSE Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

6F8G3

Kat-Nr. : CL488-66150

Synonyme

ENO2, Enolase 2, enolase 2 (gamma, neuronal), Gamma enolase, Neural enolase, Neuron specific enolase, NSE

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Galerie der Validierungsdaten

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  IF Staining of mouse brain using CL488-66150

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-66150 (NSE antibody) at dilution of 1:50.

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  IF Staining of mouse brain using CL488-66150

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-66150 (NSE antibody) at dilution of 1:50.

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  FC experiment of neuronal cells derived from human dental pulp stem using CL488-66150

  Neuronal cells derived from human dental pulp stem cells were fixed in 70% ethanol and blocked in 0.1% BSA in Staining Buffer, then stained with CoraLite® Plus 488-conjugated NSE Monoclonal Antibody (CL488-66150) (red) or unstained (blue). Image credit: @stemcell_art on Instagram

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  FC experiment of SH-SY5Y using CL488-66150

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NSE (CL488-66150, Clone:6F8G3) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66150 bindet in IF, FC (Intra) NSE und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NSE fusion protein Ag19106
• Vollständiger Name: enolase 2 (gamma, neuronal)
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC002745
• Gene symbol: ENO2
• Gene ID (NCBI): 2026
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NSE, also named as ENO2, belongs to the enolase family. Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors (PMID:7520111). ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part. And this antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C