NRAS-Specific Polyklonaler Antikörper

NRAS-Specific Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18296-1-AP

Synonyme

ALPS4, GTPase NRas, HRAS1, N ras, NRAS, NRAS1, NRAS-Specific, Transforming protein N Ras

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Galerie der Validierungsdaten

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  WB analysis using 18296-1-AP

  Recombinant fusion protein Ag27444 (KRAS-2A), Ag21645(KRAS-2B), Ag29746 (NRAS), and Ag23265(HRAS) were subjected to SDS PAGE followed by western blot with 18296-1-AP (NRAS-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 18296-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18296-1-AP (NRAS-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 18296-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 18296-1-AP (NRAS-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 18296-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 18296-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18296-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18296-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18296-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18296-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 18296-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 18296-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 18296-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 18296-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 18296-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 18296-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 18296-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 18296-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Kolonkarzinomgewebe, humanes malignes Melanomgewebe, humanes Nierengewebe, Maus-Kolongewebe, Ratten-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18296-1-AP bindet in WB, IHC, ELISA NRAS-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: neuroblastoma RAS viral (v-ras) oncogene homolog
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC005219
• Gene symbol: NRAS
• Gene ID (NCBI): 4893
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NRAS, also named as N-ras and NRAS1, is neuroblastoma RAS viral (v-ras) oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML). The antibody is specific to NRAS, can't recognize HRAS and KRAS.