NR5A1 Polyklonaler Antikörper

NR5A1 Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 18658-1-AP

Synonyme

AD4BP, Adrenal 4 binding protein, ELP, FTZ1, FTZF1, Fushi tarazu factor homolog 1, NR5A1, SF 1, SF1, SF-1, Steroid hormone receptor Ad4BP, Steroidogenic factor 1, STF 1

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Galerie der Validierungsdaten

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  WB analysis using 18658-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18658-1-AP (NR5A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 18658-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 18658-1-AP (NR5A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of A2780 using 18658-1-AP

  IP Result of anti-NR5A1 (IP:18658-1-AP, 4ug; Detection:18658-1-AP 1:300) with A2780 cells lysate 960ug.

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  IHC staining of human ovary using 18658-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 18658-1-AP (NR5A1 Antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 18658-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 18658-1-AP (NR5A1 Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 18658-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18658-1-AP (NR5A1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 18658-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18658-1-AP (NR5A1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of rat testis using 18658-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18658-1-AP (NR5A1 Antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 18658-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18658-1-AP (NR5A1 Antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HepG2 using 18658-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NR5A1 (18658-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Eierstockgewebe, Ratten-Eierstockgewebe
• Erfolgreiche IP: A2780-Zellen
• Erfolgreiche Detektion in IHC: humanes Eierstockgewebe, humanes Lebergewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 5 publications below
• IF: See 5 publications below

Produktinformation

18658-1-AP bindet in WB, IP, IHC, IF, FC, ELISA NR5A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ziege, Bisamratte (Ondatra zibethicus)
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NR5A1 fusion protein Ag13252
• Vollständiger Name: nuclear receptor subfamily 5, group A, member 1
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC032501
• Gene symbol: NR5A1
• Gene ID (NCBI): 2516
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Steroidogenic factor-1 (SF-1,STF-1), also known as NR5A1, regulates multiple genes involved in the adrenal and gonadal development and in the biosynthesis of a variety of hormones, including adrenal and gonadal steroids, anti-Mullerian hormone (AMH), and gonadotropins. SF-1 belongs to the fushi tarazu factor-1 (FTZ-F1) subfamily of orphan nuclear receptors. Initially identified as a tissue-specific transcriptional regulator of cytochrome P450 steroid hydroxylases, research studies of both global and tissue-specific knockout mice have demonstrated that SF-1 is required for the development of adrenal glands, gonads, ventromedial hypothalamus, and for the proper functioning of pituitary gonadotropes. Indeed, humans with mutations that render SF-1 transcriptionally inactive can present with testicular failure, ovarian failure, and adrenal insufficiency. Furthermore, dysregulation of SF-1 has been linked to diseases such as endometriosis and adrenocortical carcinoma.Like other nuclear hormone receptors, SF-1 has a modular domain structure composed of an N-terminal zinc finger DNA-binding domain, a ligand-binding domain, a C-terminal AF-2 activation domain, and a hinge region with AF-1-like activation activity. SF-1 also contains a fushi tarazu factor 1 box, which functions as an accessory DNA binding domain. SF-1 is primarily phosphorylated at Ser203, which is thought to enhance its transcriptional activity by promoting complex formation with transcriptional cofactors. In addition to phosphorylation at Ser203, SF-1 is subject to SUMO conjugation and acetylation at ε-amino groups of target lysine residues. Whereas SUMOylation represses SF-1 function, acetylation enhances its transcriptional activity.In the adult ovary, SF-1 localizes to theca/interstitial cells.

Publikationen

Species	Application	Title
human	IF	Elife; Induction of Sertoli-like cells from human fibroblasts by NR5A1 and GATA4.; Authors - Jianlin Liang
mouse	WB,IF	Elife; PRMT5 regulates ovarian follicle development by facilitating Wt1 translation; Authors - Min Chen
mouse	IHC	Mol Cell Endocrinol; Pde8b haploinsufficiency in mice is associated with modest adrenal defects, impaired steroidogenesis, and male infertility, unaltered by concurrent PKA or Wnt activation.; Authors - Leticia Ferro Leal
Ondatra zibethicus	IHC	J Steroid Biochem Mol Biol; Seasonal expressions of SF-1, StAR and P450scc in the scent glands of the muskrats (Ondatra zibethicus).; Authors - Wenqian Xie
mouse	IF	Toxicol Appl Pharmacol; PMID: 31175881; Authors - Jiandong Sun
mouse	WB	Toxicol Appl Pharmacol; Icariin promotes mouse Leydig cell testosterone synthesis via the Esr1/Src/Akt/Creb/Sf-1 pathway.; Authors - Jiandong Sun