NR4A1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NR4A1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

28

Kat-Nr. : 12235-1-AP

Synonyme

NGFIB, NAK1, NAK 1, N10, HMR

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Galerie der Validierungsdaten

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  WB analysis using 12235-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12235-1-AP (NR4A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12235-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12235-1-AP (NR4A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12235-1-AP

  IP result of anti-NR4A1 (IP:12235-1-AP, 4ug; Detection:12235-1-AP 1:1000) with HeLa cells lysate 1720 ug.

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  IHC staining of mouse colon using 12235-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12235-1-AP (NR4A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 12235-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12235-1-AP (NR4A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 12235-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12235-1-AP (NR4A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 12235-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12235-1-AP (NR4A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 12235-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 12235-1-AP (NR4A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COLO 320-Zellen, L02-Zellen, Mauslebergewebe, PC-3-Zellen, Rattenlebergewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maus-Kolongewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 21 publications below
• IHC: See 2 publications below
• IF: See 9 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 3 publications below

Produktinformation

12235-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA NR4A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NR4A1 fusion protein Ag2875
• Vollständiger Name: nuclear receptor subfamily 4, group A, member 1
• Berechnetes Molekulargewicht: 598 aa, 64 kDa
• Beobachtetes Molekulargewicht: 64-70 kDa
• GenBank-Zugangsnummer: BC016147
• Gene symbol: NR4A1
• Gene ID (NCBI): 3164
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NR4A1 is an orphan nuclear receptor that act concomitantly with NURR1 in regulating the expression of delay-early genes during liver regeneration. Also NR4A1 has linked not only to cell apoptosis but also proliferation in response to growth factor. It plays a role in caspase-independent cell death by activation of the ERK pathway and increased activity of MEF2 transcription factors. NR4A1 exists some isoforms with MW 65 and 34 kDa.

Publikationen

Species	Application	Title
mouse	IF	Aging (Albany NY); A systematic dissection of human primary osteoblasts in vivo at single-cell resolution.; Authors - Yun Gong
rat	WB	Sci Rep; Nur77 exacerbates PC12 cellular injury in vitro by aggravating mitochondrial impairment and endoplasmic reticulum stress.; Authors - Huimin Gao ; KD Validated
mouse	WB,IF	Sci Rep; NR4A1 Knockdown Suppresses Seizure Activity by Regulating Surface Expression of NR2B.; Authors - Yanke Zhang ; KD Validated
mouse	WB, IF	Mol Neurobiol; Contra-directional Coupling of Nur77 and Nurr1 in Neurodegeneration: A Novel Mechanism for Memantine-Induced Anti-inflammation and Anti-mitochondrial Impairment.; Authors - Xiaobo Wei
human,mouse	WB	Bioorg Chem; Structure-based design and synthesis of anti-fibrotic compounds derived from para-positioned 3,4,5-trisubstituted benzene; Authors - Wenbin Hong
human	WB	Sci Rep; The E3 ubiquitin ligase Trim13 regulates Nur77 stability via casein kinase 2α.; Authors - Bin Huang