NR2C1 Polyklonaler Antikörper

NR2C1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13658-1-AP

Synonyme

NR2C1, Orphan nuclear receptor TR2, Testicular receptor 2, TR2

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Galerie der Validierungsdaten

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  WB analysis of SH-SY5Y using 13658-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13658-1-AP (NR2C1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 13658-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13658-1-AP (NR2C1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 13658-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13658-1-AP (NR2C1 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human prostate cancer using 13658-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13658-1-AP (NR2C1 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

13658-1-AP bindet in WB, IHC, ELISA NR2C1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NR2C1 fusion protein Ag4554
• Vollständiger Name: nuclear receptor subfamily 2, group C, member 1
• Berechnetes Molekulargewicht: 467 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC040141
• Gene symbol: NR2C1
• Gene ID (NCBI): 7181
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear receptor subfamily 2 group C member 1 (NR2C1), also known as Orphan nuclear receptor TR2, is a member of the steroid/thyroid hormone receptor superfamily that controls a variety of processes, including growth, differentiation and development. TR2 is known to bind to regulatory elements of the erythropoietin gene, the muscle-specific aldolase A gene and the CNTF-15 gene. In addition to TR2, a related orphan receptor, designated TR4, has been identified. TR4 forms heterodimers with TR2, which are thought to be involved in neurogenesis and germ cell development. TR2 is known to be downregulated by both p53 and ionizing radiation, and it may play a role in linking p53 to members of the steroid recepr family.