Neuropeptide Y Polyklonaler Antikörper

Neuropeptide Y Polyklonal Antikörper für IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Rezensionen (1)

Kat-Nr. : 12833-1-AP

Synonyme

NPY, PYY4, Pro-neuropeptide Y, Neuropeptide tyrosine, CPON

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Galerie der Validierungsdaten

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  IHC staining of human brain using 12833-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12833-1-AP (Neuropeptide Y antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 12833-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12833-1-AP (Neuropeptide Y antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 12833-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12833-1-AP (Neuropeptide Y antibody) at dilution of 1:50 (under 25x lens).

• 

  IHC staining of human pancreas cancer using 12833-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12833-1-AP (Neuropeptide Y antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 12833-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12833-1-AP (Neuropeptide Y antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat brain using 12833-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12833-1-AP (Neuropeptide Y antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of rat brain using 12833-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12833-1-AP (Neuropeptide Y antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse brain using 12833-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12833-1-AP (Neuropeptide Y antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12833-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12833-1-AP (Neuropeptide Y antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 12833-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Neuropeptide Y antibody (12833-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 12833-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Neuropeptide Y antibody (12833-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse eye using 12833-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using Neuropeptide Y antibody (12833-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse eye using 12833-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using Neuropeptide Y antibody (12833-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Pankreaskarzinomgewebe, Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe, Maus-Augengewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Produktinformation

12833-1-AP bindet in WB, IHC, IF-P, ELISA Neuropeptide Y und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Neuropeptide Y fusion protein Ag3568
• Vollständiger Name: neuropeptide Y
• Berechnetes Molekulargewicht: 97 aa, 11 kDa
• GenBank-Zugangsnummer: BC029497
• Gene symbol: Neuropeptide Y
• Gene ID (NCBI): 4852
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neuropeptide Y (NPY) is one of the most abundant peptides in the mammalian central nervous system (CNS). NPY has been linked to several physiological and pathological functions, such as feeding behaviour, memory processing, pain, anxiety, cell proliferation and many other processes in the central and peripheral nervous systems. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase (MAPK), regulate intracellular calcium levels, and activate potassium channels. In addition, NPY was suggested as a potential neuroprotective agent in Alzheimer's disease by counteracting the toxic effect of β-amyloid in an in vitro model.

Publikationen

Species	Application	Title
mouse	WB	Free Radic Biol Med; Inhibition of neuronal nitric oxide synthase protects against hippocampal neuronal injuries by increasing neuropeptide Y expression in temporal lobe epilepsy mice.; Authors - Yuanyuan Yao
human	IHC	J Orthop Translat; Suprascapular nerve injury affects rotator cuff healing: A paired controlled study in a rat model.; Authors - Yucheng Sun
rat	IF	Front Behav Neurosci; Heterogeneous GAD65 Expression in Subtypes of GABAergic Neurons Across Layers of the Cerebral Cortex and Hippocampus.; Authors - Yuki Kajita
		Acta Neurobiol Exp (Wars); Aberrant changes of somatostatin and neuropeptide Y in brain of a genetic rat model for epilepsy: tremor rat.; Authors - Xiaoxue Xu
human	IHC	Acta Histochem; Loss of nerve fibers in the oviduct isthmus in women with hydrosalpinx.; Authors - Zhu Libo L
human	IHC	Acta Histochem; Decreased nerve fibers in the oviduct isthmus of women with endometriosis.; Authors - Libo Zhu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (04-12-2024)

NPY (green) worked nicely marking a subpopulation of interneurons in human brain FFPE cortex tissue.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

Neuropeptide Y Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:12833-1-AP)