NPRL2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NPRL2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 10157-1-AP

Synonyme

G21 protein, Gene 21 protein, NPRL2, tumor suppressor candidate 4, TUSC4

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 10157-1-AP

  WB result of TUSC4 antibody (10157-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TUSC4 transfected HeLa cells.

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  WB analysis of mouse pancreas using 10157-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10157-1-AP (TUSC4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse pancreas using 10157-1-AP

  IP Result of anti-TUSC4 (IP:10157-1-AP, 3ug; Detection:10157-1-AP 1:500) with mouse pancreas tissue lysate 4000ug.

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  IHC staining of human lung cancer using 10157-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 10157-1-AP (NPR2L,TUSC4 Antibody) at dilution of 1:50.

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  IHC staining of human lung cancer using 10157-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 10157-1-AP (NPR2L,TUSC4 Antibody) at dilution of 1:50.

• 

  IHC staining of human pancreas using 10157-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 10157-1-AP (NPR2L,TUSC4 Antibody) at dilution of 1:50.

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  IHC staining of human pancreas using 10157-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 10157-1-AP (NPR2L,TUSC4 Antibody) at dilution of 1:50.

• 

  IHC staining of human skeletal muscle using 10157-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 10157-1-AP (NPR2L,TUSC4 Antibody) at dilution of 1:50.

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  IHC staining of human skeletal muscle using 10157-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 10157-1-AP (NPR2L,TUSC4 Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Pankreasgewebe, HeLa-Zellen
• Erfolgreiche IP: Maus-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Pankreasgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

10157-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA NPRL2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NPRL2 fusion protein Ag0210
• Vollständiger Name: tumor suppressor candidate 4
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 44-50 kDa
• GenBank-Zugangsnummer: BC002791
• Gene symbol: TUSC4
• Gene ID (NCBI): 10641
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NPRL2 (NPR2 Like, GATOR1 Complex Subunit) is a Protein Coding gene associated with Epilepsy, Familial Focal. As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. Homologous to yeast nitrogen permease (candidate tumor suppressor) (NM_006545, BC021984) gene encodes a protein of molecular mass of 50 kDa. Data by using overlapping and nested homozygous deletions methods indicate that this protein might be a tumor suppressor involved in the development of lung cancers.

Publikationen

Species	Application	Title
human	WB, IHC	Cancer Res; TUSC4 functions as a tumor suppressor by regulating BRCA1 stability.; Authors - Yang Peng
human	WB	Mol Carcinog; Downregulation of nitrogen permease regulator like-2 activates PDK1-AKT1 and contributes to the malignant growth of glioma cells.; Authors - Ning Huang
human	WB	Cancer Sci; TUSC4/NPRL2, a novel PDK1-interacting protein, inhibits PDK1 tyrosine phosphorylation and its downstream signaling.; Authors - Kurata Atsuo A ; KD Validated
human	IHC, WB, IF, CoIP	Exp Cell Res; NPRL2 reduces the niraparib sensitivity of castration-resistant prostate cancer via interacting with UBE2M and enhancing neddylation.; Authors - Xin Zhao
human,mouse	WB,IHC	Prostate; PMID: 30178500; Authors - Zhixiong Chen
human,mouse	WB,IF	Exp Cell Res; PMID: 32234375; Authors - Shengjun Luo ; KD Validated