BNP Polyklonaler Antikörper

BNP Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(17)

Kat-Nr. : 13299-1-AP

Synonyme

Natriuretic peptides B, NPPB, BNP(3-29), BNP(2-31), BNP(1-30)

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  IHC staining of human heart using 13299-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 13299-1-AP (BNP Antibody) at dilution of 1:50.

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  IHC staining of human heart using 13299-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 13299-1-AP (BNP Antibody) at dilution of 1:50.

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  IHC staining of human brain using 13299-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 13299-1-AP (BNP Antibody) at dilution of 1:50.

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  IHC staining of human brain using 13299-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 13299-1-AP (BNP Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 13299-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BNP antibody (13299-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 3 publications below

Produktinformation

13299-1-AP bindet in IHC, IF/ICC, ELISA BNP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BNP fusion protein Ag4039
• Vollständiger Name: natriuretic peptide precursor B
• Berechnetes Molekulargewicht: 134 aa, 15 kDa
• GenBank-Zugangsnummer: BC025785
• Gene symbol: BNP
• Gene ID (NCBI): 4879
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NPPB, also known as B-type natriuretic peptide (BNP), is a cardiac hormone that is secreted mainly in the ventricles in response to increased wall stress. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. BNP is a marker of systolic and diastolic dysfunction and a strong predictor of mortality in heart failure patients.

Publikationen

Species	Application	Title
		Cell Death Dis; Asb10 accelerates pathological cardiac remodeling by stabilizing HSP70; Authors - Ke Lin
		Front Cardiovasc Med; Effects of Excessive Activation of N-methyl-D-aspartic Acid Receptors in Neonatal Cardiac Mitochondrial Dysfunction Induced by Intrauterine Hypoxia.; Authors - Yang Liu
		Pharm Biol; Epigallocatechin gallate (EGCG) attenuates myocardial hypertrophy and fibrosis induced by transverse aortic constriction via inhibiting the Akt/mTOR pathway.; Authors - Yue Cui
		Int Heart J; Phenotype and Functional Analyses in a Transgenic Mouse Model of Left Ventricular Noncompaction Caused by a DTNA Mutation.; Authors - Qing Cao
		Cell Regen; Fabrication of SA/Gel/C scaffold with 3D bioprinting to generate micro-nano porosity structure for skin wound healing: a detailed animal in vivo study.; Authors - Changmei Niu
	IHC	Front Pharmacol; Administration of USP7 inhibitor P22077 inhibited cardiac hypertrophy and remodeling in Ang II-induced hypertensive mice; Authors - Yu-Hui Gu