NOB1 Polyklonaler Antikörper

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• KD/KO Validated

NOB1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 10091-2-AP

Synonyme

NOB1P, MSTP158, MST158, EC:3.1.-.-, ART4

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Galerie der Validierungsdaten

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  WB analysis using 10091-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10091-2-AP (NOB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 10091-2-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 10091-2-AP (NOB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 10091-2-AP

  IP result of anti-NOB1 (IP:10091-2-AP, 4ug; Detection:10091-2-AP 1:300) with HepG2 cells lysate 2400ug.

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  IHC staining of human lymphoma using 10091-2-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10091-2-AP (NOB1 antibody) at dilution of 1:25 (under 10x lens).

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  IHC staining of human colon cancer using 10091-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10091-2-AP (NOB1 antibody) at dilution of 1:25 (under 10x lens).

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  IHC staining of human colon cancer using 10091-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10091-2-AP (NOB1 antibody) at dilution of 1:25 (under 40x lens).

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  IHC staining of human placenta using 10091-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10091-2-AP (NOB1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 10091-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10091-2-AP (NOB1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 10091-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using NOB1 antibody (10091-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, K-562-Zellen, SGC-7901-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe, humanes Kolonkarzinomgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:10-1:100
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below

Produktinformation

10091-2-AP bindet in WB, IHC, IF/ICC, IP, ELISA NOB1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NOB1 fusion protein Ag0141
• Vollständiger Name: NIN1/RPN12 binding protein 1 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47-50 kDa
• GenBank-Zugangsnummer: BC000050
• Gene symbol: NOB1
• Gene ID (NCBI): 28987
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NOB1 was first identified in yeast as an essential gene encoding the Nin one binding protein, which is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA. In addition, NOB1 is a crucial molecule in the maturation of the 20S proteasome and protein degradation. It serves as a chaperone to join the 20S proteasome with the 19S regulatory particle in the nucleus and facilitates the maturation of the 20S proteasome. Recently NOB1 has been reported to be overexpressed in several types of cancer, suggesting its involvement in the tumorigenesis.

Publikationen

Species	Application	Title
	WB	Nat Commun; Molecular basis for disassembly of an importin:ribosomal protein complex by the escortin Tsr2.; Authors - Sabina Schütz
	WB	Elife; A RanGTP-independent mechanism allows ribosomal protein nuclear import for ribosome assembly.; Authors - Sabina Schütz
	WB	Elife; Prefabrication of a ribosomal protein subcomplex essential for eukaryotic ribosome formation.; Authors - Cohue Peña
human	WB	World J Gastroenterol; NOB1 is essential for the survival of RKO colorectal cancer cells.; Authors - Xiao-Wen He ; KD Validated
		Oncol Rep; NOB1 silencing inhibits the growth and metastasis of laryngeal cancer cells through the regulation of JNK signaling pathway.; Authors - Xin Gao
human	WB	Oncol Lett; Effects of NOB1 on the pathogenesis of osteosarcoma and its expression on the chemosensitivity to cisplatin.; Authors - Leiming Luo ; KD Validated