NO66/C14orf169 Polyklonaler Antikörper

NO66/C14orf169 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP

Konjugation

Unkonjugiert

Kat-Nr. : 22842-1-AP

Synonyme

C14orf169, hsNO66, MAPJD, NO66, NO66/C14orf169, Nucleolar protein 66

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Galerie der Validierungsdaten

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  WB analysis using 22842-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22842-1-AP (NO66/C14orf169 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 22842-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22842-1-AP (NO66/C14orf169 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 22842-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 22842-1-AP (NO66/C14orf169 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 22842-1-AP

  IP Result of anti-NO66/C14orf169 (IP:22842-1-AP, 4ug; Detection:22842-1-AP 1:500) with Jurkat cells lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, Jurkat-Zellen, NCCIT-Zellen
• Erfolgreiche IP: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

22842-1-AP bindet in WB, IP NO66/C14orf169 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NO66/C14orf169 fusion protein Ag18721
• Vollständiger Name: chromosome 14 open reading frame 169
• Berechnetes Molekulargewicht: 641 aa, 71 kDa
• Beobachtetes Molekulargewicht: 71 kDa
• GenBank-Zugangsnummer: BC011350
• Gene symbol: C14orf169
• Gene ID (NCBI): 79697
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NO66, also known as C14orf169 and MAPJD, is a JmjC domain-containing oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. NO66 is highly conserved during evolution and shows a dual localization pattern, present in both the nucleoplasm and nucleolus (PMID: 14742713). It has been reported that NO66 participates in regulation of Osx transcriptional activity and plays an important role in osteoblast differentiation through interaction with Osx (PMID: 19927124; 23620590). Through interaction with MYC protein, NO66 plays a role in pulmonary carcinogenesis (PMID: 17308053).