NMNAT3 Polyklonaler Antikörper

NMNAT3 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 13236-1-AP

Synonyme

NaMN adenylyltransferase 3, NMNAT3, PNAT 3, PNAT3

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Galerie der Validierungsdaten

• 

  WB analysis of mouse kidney using 13236-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13236-1-AP (NMNAT3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 13236-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13236-1-AP (NMNAT3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, Maus-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

13236-1-AP bindet in WB, IHC, ELISA NMNAT3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NMNAT3 fusion protein Ag3965
• Vollständiger Name: nicotinamide nucleotide adenylyltransferase 3
• Berechnetes Molekulargewicht: 215 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24-28 kDa
• GenBank-Zugangsnummer: BC034374
• Gene symbol: NMNAT3
• Gene ID (NCBI): 349565
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Int J Biol Sci; Nicotinamide Riboside Enhances Mitochondrial Proteostasis and Adult Neurogenesis through Activation of Mitochondrial Unfolded Protein Response Signaling in the Brain of ALS SOD1G93A Mice.; Authors - Qi Zhou
mouse	WB	Food Funct; β-Nicotinamide mononucleotide supplementation prolongs the lifespan of prematurely aged mice and protects colon function in ageing mice; Authors - Yanrou Gu
mouse,rat	WB	EMBO J; Control of NAD+ homeostasis by autophagic flux modulates mitochondrial and cardiac function; Authors - Quanjiang Zhang
mouse	IHC	Int J Mol Sci; Nmnat1 Deficiency Causes Mitoribosome Excess in Diabetic Nephropathy Mediated by Transcriptional Repressor HIC1; Authors - Kazuhiro Hasegawa

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Caitlin (Verified Customer) (01-09-2020)

I used the NMNAT3 antibody for protein detection via Western Blot on mouse DRG neuronal lysate. The antibody was able to detect my protein of interest, but the bands were very faint (likely due to low protein concentration on my end).

• Applications: Western Blot,
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: mouse neurons