NMNAT1 Polyklonaler Antikörper

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• KD/KO Validated

NMNAT1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus und mehr (1)

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Rezensionen (1)

Kat-Nr. : 11399-1-AP

Synonyme

NaMN adenylyltransferase 1, NMNAT1, PNAT 1, PNAT1

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Galerie der Validierungsdaten

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  WB analysis of human testis using 11399-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11399-1-AP (NMNAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 11399-1-AP

  pig heart tissue were subjected to SDS PAGE followed by western blot with 11399-1-AP (NMNAT1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 11399-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11399-1-AP (NMNAT1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 11399-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11399-1-AP (NMNAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 11399-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11399-1-AP (NMNAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe, Hausschwein-Herzgewebe, Maushodengewebe
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Produktinformation

11399-1-AP bindet in WB, IF, IHC, ELISA NMNAT1 und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NMNAT1 fusion protein Ag1944
• Vollständiger Name: nicotinamide nucleotide adenylyltransferase 1
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 28-32 kDa
• GenBank-Zugangsnummer: BC014943
• Gene symbol: NMNAT1
• Gene ID (NCBI): 64802
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NMNAT1 is a member of the nicotinamide-nucleotide adenylyltransferases (NMNATs) which catalyze nicotinamide adenine dinucleotide (NAD) synthesis (PMID: 28445802). NMNAT is a central enzyme in NAD biosynthesis, catalyzing the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP (PMID: 17402747). NMNAT1 is widely expressed with the highest levels in skeletal muscle, heart, and kidney(PMID: 11027696). Mutations in NMNAT1 have been shown associated with the LCA9 form of the retinal degeneration pathology Leber's congenital amaurosis (PMID: 22842229, 22842230).

Publikationen

Species	Application	Title
mouse	IHC	J Am Soc Nephrol; Pre-emptive Short-term Nicotinamide Mononucleotide Treatment in a Mouse Model of Diabetic Nephropathy.; Authors - Itaru Yasuda
human,mouse	IF	Metabolism; Hyperglycemia-reduced NAD+ biosynthesis impairs corneal epithelial wound healing in diabetic mice.; Authors - Ya Li
human	WB	J Proteome Res; Reduced Nicotinamide Mononucleotide (NMNH) Potently Enhances NAD(+) and Suppresses Glycolysis, the TCA Cycle, and Cell Growth; Authors - Yan Liu
mouse	IHC	Sci Rep; Nicotinamide mononucleotide ameliorates adriamycin-induced renal damage by epigenetically suppressing the NMN/NAD consumers mediated by Twist2; Authors - Kazuhiro Hasegawa
mouse	WB	Food Funct; β-Nicotinamide mononucleotide supplementation prolongs the lifespan of prematurely aged mice and protects colon function in ageing mice; Authors - Yanrou Gu
mouse,rat	WB	EMBO J; Control of NAD+ homeostasis by autophagic flux modulates mitochondrial and cardiac function; Authors - Quanjiang Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Caitlin (Verified Customer) (09-17-2020)

I used this antibody on neuronal cell lysate for Western blotting. The antibody was able to detect the protein of interest in my lysate (NMNAT3).

• Applications: Western Blot
• Cell Tissue Type: Mouse neurons