NME1 Polyklonaler Antikörper

NME1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Rezensionen (1)

Kat-Nr. : 11086-2-AP

Synonyme

NB, Granzyme A-activated DNase, Granzyme A activated DNase, GAAD, EC:2.7.4.6

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 11086-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11086-2-AP (NME1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of multi-cells using 11086-2-AP

  WB result of 10086-2-AP (NME1 antibody).

• 

  WB analysis of A549 using 11086-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11086-2-AP (NME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11086-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11086-2-AP (NME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 11086-2-AP

  IP result of anti-NME1 (IP:11086-2-AP, 4ug; Detection:11086-2-AP 1:500) with A549 cells lysate 2800ug.

• 

  IHC staining of human lung cancer using 11086-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11086-2-AP (NME1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung cancer using 11086-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11086-2-AP (NME1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 11086-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11086-2-AP (NME1 antibody) at dilution of 1:100 (under 25x lens).

• 

  IF Staining of MCF-7 using 11086-2-AP

  Immunofluorescent analysis of MCF-7 cells using 11086-2-AP (NME1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Multizellen
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

11086-2-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA NME1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NME1 fusion protein Ag1548
• Vollständiger Name: non-metastatic cells 1, protein (NM23A) expressed in
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 16-20 kDa
• GenBank-Zugangsnummer: BC018994
• Gene symbol: NME1
• Gene ID (NCBI): 4830
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NME1 is also named as NDPKA(Nucleoside diphosphate kinase A), NM23(Metastasis inhibition factor nm23),GAAD(Granzyme A-activated DNase) and belongs to the NDK family.It is a major role in the synthesis of nucleoside triphosphates other than ATP and required for neural development including neural patterning and cell fate determination.It has 3 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Regulation of local GTP availability controls RAC1 activity and cell invasion; Authors - Anna Bianchi-Smiraglia
human	WB	Redox Biol; Regulation of metastasis suppressor NME1 by a key metabolic cofactor coenzyme A.; Authors - Bess Yi Kun Yu
human	CoIP	Int J Mol Sci; APEX2-Mediated Proximity Labeling Resolves the DDIT4-Interacting Proteome.; Authors - Marianna Naki
human	IF	J Transl Med; Identification and validation of smoking-related genes in lung adenocarcinoma using an in vitro carcinogenesis model and bioinformatics analysis.; Authors - Jin Wang
human	WB	J Proteomics; Proteomic profiling of human decidual immune proteins during Toxoplasma gondii infection.; Authors - Dan Zhang
human	IF	Oncol Rep; LINC01816 promotes the migration, invasion and epithelial‑mesenchymal transition of thyroid carcinoma cells by sponging miR‑34c‑5p and regulating CRABP2 expression levels.; Authors - Hongyuan Zhao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Chantal (Verified Customer) (10-04-2022)

Specific band appeared after 1 minute of exposure. This antibody works on mouse and human cell lines.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MEFs, Hela, HEK293FT

NME1 Antibody Western Blot validation (1:1000 dilution) in MEFs, Hela, HEK293FT (Cat no:11086-2-AP)