NMD3 Polyklonaler Antikörper

NMD3 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 16060-1-AP

Synonyme

CGI 07, hNMD3, NMD3

---

Galerie der Validierungsdaten

• 

  WB analysis using 16060-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16060-1-AP (NMD3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 16060-1-AP

  IP Result of anti-NMD3 (IP:16060-1-AP, 3ug; Detection:16060-1-AP 1:800) with HepG2 cells lysate 1720ug.

• 

  IHC staining of human breast cancer using 16060-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16060-1-AP (NMD3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16060-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16060-1-AP (NMD3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16060-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16060-1-AP (NMD3 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 16060-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16060-1-AP (NMD3 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 16060-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16060-1-AP (NMD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, L02-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

16060-1-AP bindet in WB, IP, IHC, IF, ELISA NMD3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NMD3 fusion protein Ag8894
• Vollständiger Name: NMD3 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 503 aa, 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC013317
• Gene symbol: NMD3
• Gene ID (NCBI): 51068
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human NMD3 gene encodes 60S ribosomal export protein which was found in a 60S ribosomal subunit export complex with RAN and XPO1. This nucleocytoplasmic shuttling protein NMD3 is an adaptor for export of the 60S ribosomal subunit from the nucleus. NMD3 contains a CRM-1-dependent leucine-rich nuclear export signal (NES) and a dispersed nuclear localization signal (NLS), the basic region of which is also required for nucleolar accumulation. NMD3 are required for nuclear export of the 60S ribosomal subunit in yeast and vertebrate cells, recent finding has also revealed its role in Arabidopsis thaliana.

Publikationen

Species	Application	Title
human	WB	Cell Stem Cell; HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis.; Authors - Kaosheng Lv
mouse	WB	Genes Dev; Uncoupling of GTP hydrolysis from eIF6 release on the ribosome causes Shwachman-Diamond syndrome.; Authors - Andrew J Finch
human	WB	Blood; Defective ribosome assembly in Shwachman-Diamond syndrome.; Authors - Wong Chi C CC
human	IF	Nucleic Acids Res; The induction of p53 correlates with defects in the production, but not the levels, of the small ribosomal subunit and stalled large ribosomal subunit biogenesis; Authors - Matthew John Eastham
human	WB	Cancer Lett; RNA-binding protein hnRNPU regulates multiple myeloma resistance to selinexor; Authors - Xin Wang