neuromedin B Polyklonaler Antikörper

neuromedin B Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10888-1-AP

Synonyme

neuromedin B, NMB

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Galerie der Validierungsdaten

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  WB analysis of Transfected HEK-293 using 10888-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 10888-1-AP (neuromedin B antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10888-1-AP (neuromedin B antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10888-1-AP (neuromedin B antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10888-1-AP (neuromedin B antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10888-1-AP (neuromedin B antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10888-1-AP (neuromedin B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10888-1-AP (neuromedin B antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human skin using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10888-1-AP (neuromedin B antibody) at dilution of 1:25 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hautgewebe, humanes Hirngewebe, humanes Magengewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below

Produktinformation

10888-1-AP bindet in WB, IHC, ELISA neuromedin B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Affe, Schimpanse
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: neuromedin B fusion protein Ag0842
• Vollständiger Name: neuromedin B
• Berechnetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC007407
• Gene symbol: NMB
• Gene ID (NCBI): 4828
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neuromedin B (NMB) is a bombesin-like peptide, which inhibits food intake and modulates stress-related behaviour. It exerts its function via NMB receptor (NMB-R). NMB is encoded in a 76-amino acid precursor. The mature NMB was reported to be a 10-amino-acid peptide, giving two bands of 1.2 kDa and 1.5 kDa in western blotting. NMB is expressed in brain, gastrointestinal tract, pancreas, adrenals, and adipose tissue. It regulates exocrine and endocrine secretions, cell growth, body temperature and blood pressure and glucose level in vivo.

Publikationen

Species	Application	Title
chimpanzee	IHC	Brain Struct Funct; The von Economo neurons in frontoinsular and anterior cingulate cortex in great apes and humans.; Authors - Allman John M JM
human	IHC	Cancer Lett; Neuromedin B receptor antagonist suppresses tumor angiogenesis and tumor growth in vitro and in vivo.; Authors - Park Hyun-Joo HJ
human,monkey	WB	Am J Hum Biol; Biochemical specificity of von Economo neurons in hominoids.; Authors - Stimpson Cheryl D CD