NLRX1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NLRX1 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

19

Kat-Nr. : 17215-1-AP

Synonyme

Caterpiller protein 11.3, CLR11.3, DLNB26, NLR family member X1, NLRX1, NOD26, NOD5, NOD9

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Galerie der Validierungsdaten

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  WB analysis using 17215-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17215-1-AP (NLRX1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17215-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17215-1-AP (NLRX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17215-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17215-1-AP (NLRX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 17215-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17215-1-AP (NLRX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17215-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17215-1-AP (NLRX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 17215-1-AP

  IP Result of anti-NLRX1 (IP:17215-1-AP, 3ug; Detection:17215-1-AP 1:800) with mouse heart tissue lysate 3520ug.

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  IHC staining of human heart using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse heart using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17215-1-AP (NLRX1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17215-1-AP (NLRX1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 17215-1-AP

  Immunofluorescent analysis of HepG2 cells, using NLRX1 antibody 17215-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HepG2 using 17215-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NLRX1 (17215-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Mausherzgewebe, Maus-Kolongewebe, Maus-Skelettmuskelgewebe, MCF-7-Zellen, Rattenherzgewebe, Ratten-Kolongewebe, THP-1-Zellen
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Lebergewebe, humanes Skelettmuskelgewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 18 publications below
• IHC: See 3 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

17215-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA NLRX1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NLRX1 fusion protein Ag11000
• Vollständiger Name: NLR family member X1
• Berechnetes Molekulargewicht: 975 aa, 108 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC013199
• Gene symbol: NLRX1
• Gene ID (NCBI): 79671
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Nature; Mitochondrial DNA stress primes the antiviral innate immune response.; Authors - A Phillip West
human	WB	Mol Cell; Mitochondrial protein import stress regulates the LC3 lipidation step of mitophagy through NLRX1 and RRBP1.; Authors - Samuel A Killackey ; KD Validated
human	WB,IHC	J Exp Med; NLRX1 dampens oxidative stress and apoptosis in tissue injury via control of mitochondrial activity.; Authors - Geurt Stokman ; KO Validated
human,mouse	WB	PLoS Pathog; PMID: 28542569; Authors - Jae-Hoon Kim
human	WB	Front Immunol; PMID: 30344524; Authors - Tünde Fekete
mouse	IF	Front Immunol; NLRX1 Facilitates Histoplasma capsulatum-Induced LC3-Associated Phagocytosis for Cytokine Production in Macrophages.; Authors - Juin-Hua Huang

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