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NIPSNAP3B Polyklonaler Antikörper

NIPSNAP3B Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11789-1-AP

Synonyme

NIPSNAP3, NIPSNAP3B, Protein NipSnap homolog 3B, SNAP1

Galerie der Validierungsdaten

Filter:
  • Western Blot (WB) analysis of various lysates using NIPSNAP3B Polyclonal antibody (11789-1-AP)

    WB analysis using 11789-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11789-1-AP (NIPSNAP3B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using NIPSNAP3B Polyclonal antibody (11789-1-AP)

    IHC staining of human lymphoma using 11789-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 11789-1-AP (NIPSNAP3B antibody) at dilution of 1:50 (under 10x lens).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBMauslebergewebe, Rattenlebergewebe
Erfolgreiche Detektion in IHChumanes Lymphomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunhistochemie (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 1 publications below

Produktinformation

11789-1-AP bindet in WB, IHC, ELISA NIPSNAP3B und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen NIPSNAP3B fusion protein Ag2377
Vollständiger Name nipsnap homolog 3B (C. elegans)
Berechnetes Molekulargewicht 247 aa, 28 kDa
Beobachtetes Molekulargewicht 28-30 kDa
GenBank-ZugangsnummerBC017914
Gene symbol NIPSNAP3B
Gene ID (NCBI) 55335
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NIPSNAP3B is a member of the NIPSNAP family with putative roles in vesicular trafficking (PMID: 15177564). The gene of NIPSNAP3B maps to chromosome 9q31.3, and encodes a 247-amino-acid protein with a calculated molecular mass of 28 kDa. NIPSNAP3B gene has been reported to be highly associated with low bone mineral density (BMD) variation in postmenopausal Caucasian women (PMID: 19922823).

Protokolle

Produktspezifische Protokolle
WB protocol for NIPSNAP3B antibody 11789-1-APProtokoll herunterladen
IHC protocol for NIPSNAP3B antibody 11789-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Sci Rep

The clarithromycin-binding proteins NIPSNAP1 and 2 regulate cytokine production through mitochondrial quality control

Authors - Soh Yamamoto
View Article

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Kristina (Verified Customer) (01-21-2022)

Beside NIPSNAP3A, I also tested an antibody for NIPSNAP3B as I study these proteins and their relevance in pathophysiology of Parkinson's disease for my PhD thesis. Also the NIPSNAP3B antibody showed a very precise result with one clear band.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: LUHMES