Nidogen 2 Polyklonaler Antikörper

Nidogen 2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 13530-1-AP

Synonyme

NID 2, NID2, Nidogen 2, nidogen 2 (osteonidogen), Osteonidogen

---

Galerie der Validierungsdaten

• 

  WB analysis of human placenta using 13530-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13530-1-AP (Nidogen 2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13530-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13530-1-AP (Nidogen 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 13530-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13530-1-AP (Nidogen 2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 13530-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13530-1-AP (Nidogen 2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 13530-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13530-1-AP (Nidogen 2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse skeletal muscle using 13530-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13530-1-AP (Nidogen 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 13530-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13530-1-AP (Nidogen 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13530-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13530-1-AP (Nidogen 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13530-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13530-1-AP (Nidogen 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, humanes Herzgewebe, humanes Plazenta-Gewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, Mausnierengewebe, Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

13530-1-AP bindet in WB, IHC, ELISA Nidogen 2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Nidogen 2 fusion protein Ag4424
• Vollständiger Name: nidogen 2 (osteonidogen)
• Berechnetes Molekulargewicht: 1375 aa, 151 kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: BC035608
• Gene symbol: NID2
• Gene ID (NCBI): 22795
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nidogen, also referred to as entactin, represents a highly conserved protein in vertebrates and invertebrates and is found in almost all basement membranes (BM). Nidogen-1 (150 kDa) and nidogen-2 (200 kDa) are elongated molecules composed of three globular domains (G1, G2, and G3) connected by a flexible, protease-sensitive link and a rigid rod-like domain. Western blot analysis of protein extracts of heart, lung, and skeletal muscle of mice, major bands of about 200 kDa were detected, accompanied by smaller and variable bands of 160-170 kDa. (PMID: 12215539)

Publikationen

Species	Application	Title
human	IHC	Front Cell Dev Biol; Time-Resolved Extracellular Matrix Atlas of the Developing Human Skin Dermis.; Authors - Mansheng Li